ArticleMolecular biology of the cell2005
Time-resolved imaging of HIV-1 Env-mediated lipid and content mixing between a single virion and cell membrane.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 69 citations in OpenAlex.
- Article
- Article
- Endosomes supporting fusion mediated by vesicular stomatitis virus glycoprotein have distinctive motion and acidification.Traffic (Copenhagen, Denmark) · 2022Article
- The late endosome-resident lipid bis(monoacylglycero)phosphate is a cofactor for Lassa virus fusion.PLoS pathogens · 2021Article
- Review
- Single-Molecule Super-Resolution Imaging of T-Cell Plasma Membrane CD4 Redistribution upon HIV-1 Binding.Viruses · 2021Article
- A biophysical perspective on receptor-mediated virus entry with a focus on HIV.Biochimica et biophysica acta. Biomembranes · 2020Review
- Fluorescence Microscopy of the HIV-1 Envelope.Viruses · 2020Review
- Single-cell glycolytic activity regulates membrane tension and HIV-1 fusion.PLoS pathogens · 2020Article
- Antiviral peptides as promising therapeutic drugs.Cellular and molecular life sciences : CMLS · 2019Review
- Single Virion Tracking Microscopy for the Study of Virus Entry Processes in Live Cells and Biomimetic Platforms.Advances in experimental medicine and biology · 2019Review
- Vesicular stomatitis virus G protein transmembrane region is crucial for the hemi-fusion to full fusion transition.Scientific reports · 2018Article
- Six-helix bundle completion in the distal C-terminal heptad repeat region of gp41 is required for efficient human immunodeficiency virus type 1 infection.Retrovirology · 2018Article
- An improved labeling strategy enables automated detection of single-virus fusion and assessment of HIV-1 protease activity in single virions.The Journal of biological chemistry · 2017Article
- Switching between Successful and Dead-End Intermediates in Membrane Fusion.International journal of molecular sciences · 2017Article
- Effects of HIV-1 gp41-Derived Virucidal Peptides on Virus-like Lipid Membranes.Biophysical journal · 2017Article
- Functional organization of the HIV lipid envelope.Scientific reports · 2016Article
- Dynamics and restriction of murine leukemia virus cores in mitotic and interphase cells.Retrovirology · 2015Article
- Distinct requirements for HIV-cell fusion and HIV-mediated cell-cell fusion.The Journal of biological chemistry · 2015Article
- Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
A method has been developed to follow fusion of individual pseudotyped virus expressing HIV-1 Env to cells by time-resolved fluorescence microscopy. Viral envelopes were labeled with a fluorescent lipid dye (DiD) and virus content was rendered visible by incorporating a Gag-GFP chimera. The Gag-GFP is naturally cleaved to the much smaller NC-GFP fragment in the mature virions. NC-GFP was readily released upon permeabilization of the viral envelope, whereas the capsid was retained. The NC-GFP thus provides a relatively small and mobile aqueous marker to follow viral content transfer. In fusion experiments, virions were bound to cells at low temperature, and fusion was synchronously triggered by a temperature jump. DiD transferred from virions to cells without a significant lag after the temperature jump. Some virions released DiD but retained NC-GFP. Surprisingly, the fraction of lipid mixing events yielding NC-GFP transfer was dependent on the type of target cell: of three infectable cell lines, only one permitted NC-GFP transfer within minutes of raising temperature. NC-GFP release did not correlate with the level of CD4 or coreceptor expression in the target cells. The data indicate that fusion pores formed by HIV-1 Env can remain small for a relatively long time before they enlarge.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.