ArticleMolecular biology of the cell2005
Alternative splicing of SNAP-25 regulates secretion through nonconservative substitutions in the SNARE domain.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 78 citations in OpenAlex.
- The Association of SNAP25 Gene Polymorphisms in Attention Deficit/Hyperactivity Disorder: a Systematic Review and Meta-Analysis.Molecular neurobiology · 2017Pooled it
- Butterfly brains change in morphology and in gene splicing patterns after brief pheromone exposure.BMC biology · 2026Article
- Isoswitching drives the aging process in human brains.bioRxiv : the preprint server for biology · 2025Article
- Multiple structural states in an intrinsically disordered protein, SNAP-25, using circular dichroism.Biophysical journal · 2025Article
- The mesoscale organization of syntaxin 1A and SNAP25 is determined by SNARE-SNARE interactions.eLife · 2021Article
- Cerebrospinal Fluid Biomarkers of Alzheimer's Disease: Current Evidence and Future Perspectives.Brain sciences · 2021Review
- Developmental Time Course of SNAP-25 Isoforms Regulate Hippocampal Long-Term Synaptic Plasticity and Hippocampus-Dependent Learning.International journal of molecular sciences · 2020Article
- Membrane Fusion Involved in Neurotransmission: Glimpse from Electron Microscope and Molecular Simulation.Frontiers in molecular neuroscience · 2017Review
- Regulation of Ca2+ channels by SNAP-25 via recruitment of syntaxin-1 from plasma membrane clusters.Molecular biology of the cell · 2016Article
- Quantitative mass spectrometry reveals changes in SNAP-25 isoforms in schizophrenia.Schizophrenia research · 2016Article
- SNARE complex in developmental psychiatry: neurotransmitter exocytosis and beyond.Journal of neural transmission (Vienna, Austria : 1996) · 2016Review
- SNAP25 ameliorates sensory deficit in rats with spinal cord transection.Molecular neurobiology · 2014Article
- NFAT1 and NFAT3 cooperate with HDAC4 during regulation of alternative splicing of PMCA isoforms in PC12 cells.PloS one · 2014Article
- Synaptotagmin interaction with SNAP-25 governs vesicle docking, priming, and fusion triggering.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Role of presynaptic metabotropic glutamate receptors in the induction of long-term synaptic plasticity of vesicular release.Neuropharmacology · 2013Review
- Function of alternative splicing.Gene · 2013Review
- SNARE requirements en route to exocytosis: from many to few.Journal of molecular neuroscience : MN · 2012Review
- Presynaptic residual calcium and synaptic facilitation at hippocampal synapses of mice with altered expression of SNAP-25.Brain research · 2012Article
- Alternative splicing of the human gene SYBL1 modulates protein domain architecture of Longin VAMP7/TI-VAMP, showing both non-SNARE and synaptobrevin-like isoforms.BMC molecular biology · 2011Article
- Regional and developmental brain expression patterns of SNAP25 splice variants.BMC neuroscience · 2011Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
The essential membrane fusion apparatus in mammalian cells, the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex, consists of four alpha-helices formed by three proteins: SNAP-25, syntaxin 1, and synaptobrevin 2. SNAP-25 contributes two helices to the complex and is targeted to the plasma membrane by palmitoylation of four cysteines in the linker region. It is alternatively spliced into two forms, SNAP-25a and SNAP-25b, differing by nine amino acids substitutions. When expressed in chromaffin cells from SNAP-25 null mice, the isoforms support different levels of secretion. Here, we investigated the basis of that different secretory phenotype. We found that two nonconservative substitutions in the N-terminal SNARE domain and not the different localization of one palmitoylated cysteine cause the functional difference between the isoforms. Biochemical and molecular dynamic simulation experiments revealed that the two substitutions do not regulate secretion by affecting the property of SNARE complex itself, but rather make the SNAP-25b-containing SNARE complex more available for the interaction with accessory factor(s).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.