ArticleMolecular biology of the cell2005
Differential light chain assembly influences outer arm dynein motor function.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- IC2 participates in the cooperative activation of outer arm dynein densely attached to microtubules.Cell structure and function · 2023Article
- Light chain 2 is a Tctex-type related axonemal dynein light chain that regulates directional ciliary motility in Trypanosoma brucei.PLoS pathogens · 2022Article
- Consensus nomenclature for dyneins and associated assembly factors.The Journal of cell biology · 2022Article
- Structure of a microtubule-bound axonemal dynein.Nature communications · 2021Article
- E-hooks provide guidance and a soft landing for the microtubule binding domain of dynein.Scientific reports · 2018Article
- Ciliary Motility: Regulation of Axonemal Dynein Motors.Cold Spring Harbor perspectives in biology · 2017Review
- Structure and function of outer dynein arm intermediate and light chain complex.Molecular biology of the cell · 2016Article
- The awesome power of dikaryons for studying flagella and basal bodies in Chlamydomonas reinhardtii.Cytoskeleton (Hoboken, N.J.) · 2014Review
- Protein-protein interactions between intermediate chains and the docking complex of Chlamydomonas flagellar outer arm dynein.FEBS letters · 2013Article
- TCTEX1D4, a novel protein phosphatase 1 interactor: connecting the phosphatase to the microtubule network.Biology open · 2013Article
- Article
- Integrated control of axonemal dynein AAA(+) motors.Journal of structural biology · 2012Review
- The versatile molecular complex component LC8 promotes several distinct steps of flagellar assembly.The Journal of cell biology · 2012Article
- Article
- Structure-function analysis of dynein light chain 1 identifies viable motility mutants in bloodstream-form Trypanosoma brucei.Eukaryotic cell · 2011Article
- Novel LC8 mutations have disparate effects on the assembly and stability of flagellar complexes.The Journal of biological chemistry · 2009Article
- Article
- Three members of the LC8/DYNLL family are required for outer arm dynein motor function.Molecular biology of the cell · 2008Article
- Partially functional outer-arm dynein in a novel Chlamydomonas mutant expressing a truncated gamma heavy chain.Eukaryotic cell · 2008Article
- Dyneins across eukaryotes: a comparative genomic analysis.Traffic (Copenhagen, Denmark) · 2007Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Tctex1 and Tctex2 were originally described as potential distorters/sterility factors in the non-Mendelian transmission of t-haplotypes in mice. These proteins have since been identified as subunits of cytoplasmic and/or axonemal dyneins. Within the Chlamydomonas flagellum, Tctex1 is a subunit of inner arm I1. We have now identified a second Tctex1-related protein (here termed LC9) in Chlamydomonas. LC9 copurifies with outer arm dynein in sucrose density gradients and is missing only in those strains completely lacking this motor. Zero-length cross-linking of purified outer arm dynein indicates that LC9 interacts directly with both the IC1 and IC2 intermediate chains. Immunoblot analysis revealed that LC2, LC6, and LC9 are missing in an IC2 mutant strain (oda6-r88) that can assemble outer arms but exhibits significantly reduced flagellar beat frequency. This defect is unlikely to be due to lack of LC6, because an LC6 null mutant (oda13) exhibits only a minor swimming abnormality. Using an LC2 null mutant (oda12-1), we find that although some outer arm dynein components assemble in the absence of LC2, they are nonfunctional. In contrast, dyneins from oda6-r88, which also lack LC2, retain some activity. Furthermore, we observed a synthetic assembly defect in an oda6-r88 oda12-1 double mutant. These data suggest that LC2, LC6, and LC9 have different roles in outer arm assembly and are required for wild-type motor function in the Chlamydomonas flagellum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.