ArticleThe Biochemical journal2006
Identification of a mitochondrial transporter for pyrimidine nucleotides in Saccharomyces cerevisiae: bacterial expression, reconstitution and functional characterization.
Article in The Biochemical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 81 citations in OpenAlex.
- Mitochondria in the middle of inter- organellar cross talk: insights from yeast to humans.Frontiers in cell and developmental biology · 2026Review
- Biogenesis and function of the mitochondrial solute carrier (SLC25) family in yeast.Biological chemistry · 2025Review
- High tide or low tide: the transport and metabolism of mitochondrial nucleotides.The Biochemical journal · 2025Review
- Human RCC1L is involved in the maintenance of mitochondrial nucleoids and mtDNA.Scientific reports · 2025Article
- Article
- Minimal Out-of-Equilibrium Metabolism for Synthetic Cells: A Membrane Perspective.ACS synthetic biology · 2023Review
- Mitochondrial transport and metabolism of the vitamin B-derived cofactors thiamine pyrophosphate, coenzyme A, FAD and NADIUBMB life · 2022Review
- Evidence for Non-Essential Salt Bridges in the M-Gates of Mitochondrial Carrier Proteins.International journal of molecular sciences · 2022Article
- The intra-mitochondrial O-GlcNAcylation system rapidly modulates OXPHOS function and ROS release in the heart.Communications biology · 2022Article
- Learning from Yeast about Mitochondrial Carriers.Microorganisms · 2021Review
- Uridine Treatment of the First Known Case of SLC25A36 Deficiency.International journal of molecular sciences · 2021Article
- Mitochondrial Carriers and Substrates Transport Network: A Lesson fromInternational journal of molecular sciences · 2021Review
- Tricarboxylate Citrate Transporter of an Oleaginous FungusFrontiers in nutrition · 2021Article
- Molecular Mechanism of Citrate Efflux by the Mitochondrial Citrate Transporter CT in Filamentous FungusFrontiers in microbiology · 2021Article
- Harnessing sub-organelle metabolism for biosynthesis of isoprenoids in yeast.Synthetic and systems biotechnology · 2020Review
- Review
- Metabolic Roles of Plant Mitochondrial Carriers.Biomolecules · 2020Review
- Splitting the functions of Rim2, a mitochondrial iron/pyrimidine carrier.Mitochondrion · 2019Article
- Uncoupling proteins 1 and 2 (UCP1 and UCP2) fromThe Journal of biological chemistry · 2018Article
- Mitochondrial ATP-Mg/phosphate carriers transport divalent inorganic cations in complex with ATP.Journal of bioenergetics and biomembranes · 2017Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pyrimidine (deoxy)nucleoside triphosphates are required in mitochondria for the synthesis of DNA and the various types of RNA present in these organelles. In Saccharomyces cerevisiae, these nucleotides are synthesized outside the mitochondrial matrix and must therefore be transported across the permeability barrier of the mitochondrial inner membrane. However, no protein has ever been found to be associated with this transport activity. In the present study, Rim2p has been identified as a yeast mitochondrial pyrimidine nucleotide transporter. Rim2p (replication in mitochondria 2p) is a member of the mitochondrial carrier protein family having some special features. The RIM2 gene was overexpressed in bacteria. The purified protein was reconstituted into liposomes and its transport properties and kinetic parameters were characterized. It transported the pyrimidine (deoxy)nucleoside tri- and di-phosphates and, to a lesser extent, pyrimidine (deoxy)nucleoside monophosphates, by a counter-exchange mechanism. Transport was saturable, with an apparent K(m) of 207 microM for TTP, 404 microM for UTP and 435 microM for CTP. Rim2p was strongly inhibited by mercurials, bathophenanthroline, tannic acid and Bromocresol Purple, and partially inhibited by bongkrekic acid. Furthermore, the Rim2p-mediated heteroexchanges, TTP/TMP and TTP/TDP, are electroneutral and probably H+-compensated. The main physiological role of Rim2p is proposed to be to transport (deoxy)pyrimidine nucleoside triphosphates into mitochondria in exchange for intramitochondrially generated (deoxy)pyrimidine nucleoside monophosphates.
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