ArticleBiophysical journal2005
Yeast kinetochore microtubule dynamics analyzed by high-resolution three-dimensional microscopy.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Asymmetric centromere and gene locus positioning inbioRxiv : the preprint server for biology · 2025Article
- Asymmetric chromatin retention and nuclear envelopes separate chromosomes in fused cells in vivo.Communications biology · 2022Article
- Designing a rigorous microscopy experiment: Validating methods and avoiding bias.The Journal of cell biology · 2019Review
- Microtubule dynamics regulation reconstituted in budding yeast lysates.Journal of cell science · 2018Article
- Changes in single-molecule integrin dynamics linked to local cellular behavior.Molecular biology of the cell · 2016Article
- DNA damage signalling targets the kinetochore to promote chromatin mobility.Nature cell biology · 2016Article
- Chromosome domain architecture and dynamic organization of the fission yeast genome.FEBS letters · 2015Review
- Article
- Budding yeast kinetochore proteins, Chl4 and Ctf19, are required to maintain SPB-centromere proximity during G1 and late anaphase.PloS one · 2014Article
- Article
- S. cerevisiae chromosomes biorient via gradual resolution of syntely between S phase and anaphase.Cell · 2013Article
- Bub1 kinase and Sgo1 modulate pericentric chromatin in response to altered microtubule dynamics.Current biology : CB · 2012Article
- Mutations in the nucleotide binding pocket of MreB can alter cell curvature and polar morphology in Caulobacter.Molecular microbiology · 2011Article
- Principles of chromosomal organization: lessons from yeast.The Journal of cell biology · 2011Review
- Dynamic macrophage "probing" is required for the efficient capture of phagocytic targets.The Journal of cell biology · 2010Article
- A small GTPase molecular switch regulates epigenetic centromere maintenance by stabilizing newly incorporated CENP-A.Nature cell biology · 2010Article
- Advanced hardware and software tools for fast multidimensional imaging of living cells.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Cytoskeletal dynamics in fission yeast: a review of models for polarization and division.HFSP journal · 2010Article
- Kinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerases.The Journal of cell biology · 2010Article
- Computational image analysis of cellular dynamics: a case study based on particle tracking.Cold Spring Harbor protocols · 2009Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
We have probed single kinetochore microtubule (k-MT) dynamics in budding yeast in the G1 phase of the cell cycle by automated tracking of a green fluorescent protein tag placed proximal to the centromere on chromosome IV and of a green fluorescent protein tag fused to the spindle pole body protein Spc42p. Our method reliably distinguishes between different dynamics in wild-type and mutant strains and under different experimental conditions. Using our methods we established that in budding yeast, unlike in metazoans, chromosomes make dynamic attachments to microtubules in G1. This makes it possible to interpret measurements of centromere tag dynamics as reflecting k-MT dynamics. We have examined the sensitivity of our assay by studying the effect of temperature, exposure to benomyl, and a tubulin mutation on k-MT dynamics. We have found that lowering the temperature and exposing cells to benomyl attenuate k-MT dynamics in a similar manner. We further observe that, in contrast to previous reports, the mutant tub2-150 forms k-MTs that depolymerize faster than wild type. Based on these findings, we propose high-resolution light microscopy of centromere dynamics in G1 yeast cells as a sensitive assay for the regulation of single k-MT dynamics.
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