ArticleJournal of clinical pathology2005
A practical approach to the clinical diagnosis of Ewing's sarcoma/primitive neuroectodermal tumour and other small round cell tumours sharing EWS rearrangement using new fluorescence in situ hybridisation probes for EWSR1 on formalin fixed, paraffin wax embedded tissue.
Article in Journal of clinical pathology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 74 citations in OpenAlex.
- Primary desmoplastic small round cell tumor of the submandibular gland: a case report and literature review.Diagnostic pathology · 2022Review
- Mediastinal desmoplastic small round cell tumor.Medicine · 2020Article
- Article
- A Nonpediatric Extraosseous Ewing Sarcoma of the Pancreas: Differential Diagnosis and Therapeutic Strategies.Case reports in oncological medicine · 2020Article
- Renal primitive neuroectodermal tumor with elevated plasma adrenocorticotropic hormone levels: A case report.IJU case reports · 2019Article
- Primary bronchial Ewing sarcoma.International journal of surgery case reports · 2019Article
- Myxoid liposarcoma with cartilaginous differentiation showingOncology letters · 2017Article
- Desmoplastic small round cell tumor: evaluation of reverse transcription-polymerase chain reaction and fluorescence in situ hybridization as ancillary molecular diagnostic techniques.Virchows Archiv : an international journal of pathology · 2017Article
- Cytogenetically confirmed primary Ewing's sarcoma of the pancreas.BMJ case reports · 2017Article
- Diagnosis of desmoplastic small-round-cell tumor by cytogenetic analysis: a case report.Clinical case reports · 2016Article
- Ewing's Sarcoma as a Second Malignancy in Long-Term Survivors of Childhood Hematologic Malignancies.Sarcoma · 2016Article
- A peripheral primitive neuroectodermal tumor originating from the pancreas: a case report and review of the literature.Surgical case reports · 2015Article
- Hyalinizing clear cell carcinoma with EWSR1-ATF1 fusion gene: report of three cases with molecular analyses.Virchows Archiv : an international journal of pathology · 2015Article
- Clinicopathological and molecular spectrum of ewing sarcomas/PNETs, including validation of EWSR1 rearrangement by conventional and array FISH technique in certain cases.Pathology oncology research : POR · 2014Article
- Molecular strategies for detecting chromosomal translocations in soft tissue tumors (review).International journal of molecular medicine · 2014Review
- Two Cases of Spinal, Extraosseous, Intradural Ewing's sarcoma/Peripheral Neuroectodermal Tumor: Radiologic, Pathologic, and Molecular Analysis.Journal of clinical imaging science · 2014Article
- Usefulness of a monoclonal ERG/FLI1 antibody for immunohistochemical discrimination of Ewing family tumors.American journal of clinical pathology · 2013Article
- Paratesticular desmoplastic small round cell tumour: an unusual tumour with an unusual fusion; cytogenetic and molecular genetic analysis combining RT-PCR and COBRA-FISH.Clinical sarcoma research · 2012Article
- Histological heterogeneity of Ewing's sarcoma/PNET: an immunohistochemical analysis of 415 genetically confirmed cases with clinical support.Virchows Archiv : an international journal of pathology · 2009Article
- Analysis of CHOP rearrangement in pleomorphic liposarcomas using fluorescence in situ hybridization.Cancer science · 2009Article
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOver 90% of Ewing's sarcoma/primitive neuroectodermal tumour (ES/PNET) cases have the t(11;22) chromosomal rearrangement, which is also found in other small round cell tumours, including desmoplastic small round cell tumour (DSRCT) and clear cell sarcoma (CCS). Although this rearrangement can be analysed by fluorescence in situ hybridisation (FISH) using routinely formalin fixed, paraffin wax embedded (FFPE) tissues when fresh or frozen tissues are not available, a sensitive and convenient detection method is needed for routine clinical diagnosis.
aimsTo investigate the usefulness of newly developed probes for detecting EWS rearrangement resulting from chromosomal translocations using FISH and FFPE tissue in the clinical diagnosis of ES/PNET, DSRCT, and CCS.
methodsSixteen ES/PNETs, six DSRCTs, and six CCSs were studied. Three poorly differentiated synovial sarcomas, three alveolar rhabdomyosarcomas, and three neuroblastomas served as negative controls. Interphase FISH analysis was performed on FFPE tissue sections with a commercially available EWSR1 (22q12) dual colour, breakapart rearrangement probe.
resultsOne fused signal and one split signal of orange and green, demonstrating rearrangement of the EWS gene, was detected in 14 of 16 ES/PNETs, all six DRSCTs, and five of six CCSs, but not in the negative controls.
conclusionsInterphase FISH using this newly developed probe is sensitive and specific for detecting the EWS gene on FFPE tissues and is of value in the routine clinical diagnosis of ES/PNET, DSRCT, and CCS.
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