ArticleNeurochemical research
Effect of hyperosmotic conditions on the expression of the betaine-GABA-transporter (BGT-1) in cultured mouse astrocytes.
Article in Neurochemical research. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- Astrocytes: GABAceptive and GABAergic Cells in the Brain.Frontiers in cellular neuroscience · 2022Review
- Impaired Expression of GABA Signaling Components in the Alzheimer's Disease Middle Temporal Gyrus.International journal of molecular sciences · 2020Article
- Pharmacological Characterization of a Betaine/GABA Transporter 1 (BGT1) Inhibitor Displaying an Unusual Biphasic Inhibition Profile and Anti-seizure Effects.Neurochemical research · 2020Article
- Physiology of Astroglia.Physiological reviews · 2018Review
- Inhibition of GABA transporters fails to afford significant protection following focal cerebral ischemia.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2018Article
- The betaine/GABA transporter and betaine: roles in brain, kidney, and liver.Frontiers in physiology · 2014Review
- Methodological limitations in determining astrocytic gene expression.Frontiers in endocrinology · 2013Review
- Mapping and signaling of neural pathways involved in the regulation of hydromineral homeostasis.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2013Review
- Recognition of betaine as an inhibitor of lipopolysaccharide-induced nitric oxide production in activated microglial cells.Iranian biomedical journal · 2012Article
- Effects of betaine on lipopolysaccharide-induced memory impairment in mice and the involvement of GABA transporter 2.Journal of neuroinflammation · 2011Article
- Functional expression of γ-amino butyric acid transporter 2 in human and guinea pig airway epithelium and smooth muscle.American journal of respiratory cell and molecular biology · 2011Article
- Deletion of the betaine-GABA transporter (BGT1; slc6a12) gene does not affect seizure thresholds of adult mice.Epilepsy research · 2011Article
- Choline and betaine in health and disease.Journal of inherited metabolic disease · 2011Review
- SNAT2 amino acid transporter is regulated by amino acids of the SLC6 gamma-aminobutyric acid transporter subfamily in neocortical neurons and may play no role in delivering glutamine for glutamatergic transmission.The Journal of biological chemistry · 2009Article
- Replica-moulded polydimethylsiloxane culture vessel lids attenuate osmotic drift in long-term cell cultures.Journal of biosciences · 2009Article
- Inhibition of the betaine-GABA transporter (mGAT2/BGT-1) modulates spontaneous electrographic bursting in the medial entorhinal cortex (mEC).Epilepsy research · 2008Article
- Hyperosmotic stress response: comparison with other cellular stresses.Pflugers Archiv : European journal of physiology · 2007Review
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The adaptation of cells to hyperosmotic conditions involves accumulation of organic osmolytes to achieve osmotic equilibrium and maintenance of cell volume. The Na+ and Cl(-)-coupled betaine/GABA transporter, designated BGT-1, is responsible for the cellular accumulation of betaine and has been proposed to play a role in osmoregulation in the brain. BGT-1 is also called GAT2 (GABA transporter 2) when referring to the mouse transporter homologue. Using Western Blotting the expression of the mouse GAT2 protein was investigated in astrocyte primary cultures exposed to a growth medium made hyperosmotic (353+/-2.5 mosmol/kg) by adding sodium chloride. A polyclonal anti-BGT-1 antibody revealed the presence of two characteristic bands at 69 and 138 kDa. When astrocytes were grown for 24 h under hyperosmotic conditions GAT2 protein was up-regulated 2-4-fold compared to the level of the isotonic control. Furthermore, the expected dimer of GAT2 was also up-regulated after 24 h under the hyperosmotic conditions. The [3H]GABA uptake was examined in the hyperosmotic treated astrocytes, and characterized using different selective GABA transport inhibitors. The up-regulation of GAT2 protein was not affecting total GABA uptake but the hyperosmotic condition did change total GABA uptake possibly involving GAT1. Immunocytochemical studies revealed cell membrane localization of GAT2 throughout astroglial processes. Taken together, these results indicate that astroglial GAT2 expression and function may be regulated by hyperosmolarity in cultured mouse astrocytes, suggesting a role of GAT2 in osmoregulation in neural cells.
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