ArticleNeurochemical research
BMP enhances transcriptional responses to NGF during PC12 cell differentiation.
Article in Neurochemical research. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Physical Stimulation Methods Developed for In Vitro Neuronal Differentiation Studies of PC12 Cells: A Comprehensive Review.International journal of molecular sciences · 2024Review
- SP600125 Enhances Temperature-Controlled Repeated Thermal Stimulation-Induced Neurite Outgrowth in PC12-P1F1 Cells.International journal of molecular sciences · 2022Article
- Characterization of PC12 Cell Subclones with Different Sensitivities to Programmed Thermal Stimulation.International journal of molecular sciences · 2020Article
- Role for Egr1 in the Transcriptional Program Associated with Neuronal Differentiation of PC12 Cells.PloS one · 2017Article
- BMP signaling in axon regeneration.Current opinion in neurobiology · 2014Review
- The ubiquitin ligase Praja1 reduces NRAGE expression and inhibits neuronal differentiation of PC12 cells.PloS one · 2013Article
- Gene expression pattern in PC12 cells with reduced PMCA2 or PMCA3 isoform: selective up-regulation of calmodulin and neuromodulin.Molecular and cellular biochemistry · 2012Article
- Global expression analysis identified a preferentially nerve growth factor-induced transcriptional program regulated by sustained mitogen-activated protein kinase/extracellular signal-regulated kinase (ERK) and AP-1 protein activation during PC12 cell differentiation.The Journal of biological chemistry · 2011Article
- Transcriptional responses of cultured rat sympathetic neurons during BMP-7-induced dendritic growth.PloS one · 2011Article
- Article
- Axotomy-induced neurotrophic withdrawal causes the loss of phenotypic differentiation and downregulation of NGF signalling, but not death of septal cholinergic neurons.Molecular neurodegeneration · 2010Article
- Are BMPs involved in normal nerve and following transection?: a pilot study.Clinical orthopaedics and related research · 2009Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bone morphogenetic proteins (BMPs) enhance neurite outgrowth in nerve growth factor (NGF)-stimulated PC12 cells. To investigate the mechanism of this potentiating effect, real-time PCR was used to analyze the expression of 45 selected genes. A robust increase in expression of 10 immediate early genes including Egr1-4, Hes1, Junb, Jun and Fos was observed already after 1 h treatment with NGF alone. NGF plus BMP4 further increased these transcripts at 1 h and activated 18 additional genes. BMP4 alone induced Smad6, Mtap1b and Hes1. Egr3 was the gene most strongly upregulated by NGF and BMP4. However, luciferase assays showed that the cloned Egr3 proximal promoter was not involved in the BMP4 potentiation. Blocking Egr3 and Junb function by dominant-negative constructs reduced neurite outgrowth under stimulating conditions, proving that activation of members of both the Egr and Jun families is necessary for maximal PC12 cell response to NGF and BMP4.
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