ArticleEuropean biophysics journal : EBJ2006
Reconstitution and alignment by a magnetic field of a beta-barrel membrane protein in bicelles.
Article in European biophysics journal : EBJ, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- ChronicJournal of clinical medicine · 2024Article
- Supramolecular Organization of Apolipoprotein-A-I-Derived Peptides within Disc-like Arrangements.Biophysical journal · 2018Article
- Moderate and strong static magnetic fields directly affect EGFR kinase domain orientation to inhibit cancer cell proliferation.Oncotarget · 2016Article
- Systematic analysis of the use of amphipathic polymers for studies of outer membrane proteins using mass spectrometry.International journal of mass spectrometry · 2015Article
- A Practical Implicit Membrane Potential for NMR Structure Calculations of Membrane Proteins.Biophysical journal · 2015Article
- The magic of bicelles lights up membrane protein structure.Chemical reviews · 2012Review
- Expression, refolding, and initial structural characterization of the Y. pestis Ail outer membrane protein in lipids.Biochimica et biophysica acta · 2011Article
- Recent Advances in the Application of Solution NMR Spectroscopy to Multi-Span Integral Membrane Proteins.Progress in nuclear magnetic resonance spectroscopy · 2009Article
- Orientation of the Escherichia coli outer membrane protein OmpX in phospholipid bilayer membranes determined by solid-State NMR.Biochemistry · 2008Article
- Solution NMR of membrane proteins in bilayer mimics: small is beautiful, but sometimes bigger is better.Biochimica et biophysica acta · 2007Review
- NMR structural studies of the bacterial outer membrane protein OmpX in oriented lipid bilayer membranes.Biochimica et biophysica acta · 2007Article
- Isotropic bicelles stabilize the functional form of a small multidrug-resistance pump for NMR structural studies.Journal of the American Chemical Society · 2007Article
- Effects of lipid chain length and unsaturation on bicelles stability. A phosphorus NMR study.Biophysical journal · 2006Article
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Authors and funding
5 authors.
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Abstract
A protocol is described for the reconstitution of a transmembrane beta-barrel protein domain, tOmpA, into lipid bicelles. tOmpA is the largest protein to be reconstituted in bicelles to date. Its insertion does not prevent bicelles from orienting with their plane either parallel or perpendicular to the magnetic field, depending on the absence or presence of paramagnetic ions. In the latter case, tOmpA is shown to align with the axis of the beta-barrel parallel to the magnetic field, i.e. perpendicular to the plane of the bilayer, an orientation conforming to that in natural membranes and favourable to structural studies by solid-state NMR. Reconstitution into bicelles may offer an interesting approach for structural studies of membrane proteins in a medium resembling a biological membrane, using either NMR or other biophysical techniques. Our data suggest that alignment in the magnetic field of membrane proteins included into bicelles may be facilitated if the protein is folded as a beta-barrel structure.
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