ArticleHistochemistry and cell biology2005
Epithelial vs myofibroblast differentiation in immortal rat lung cell lines--modulating effects of bleomycin.
Article in Histochemistry and cell biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed.
- mRNA Levels of Epithelial and Mesenchymal Markers in Lung Epithelial Cell Lines.Reports of biochemistry & molecular biology · 2023Article
- Mechanisms and modulation of microvesicle uptake in a model of alveolar cell communication.The Journal of biological chemistry · 2017Article
- Fluid-induced lung injury-role of TRPV4 channels.Pflugers Archiv : European journal of physiology · 2017Article
- Alveolar Epithelial Cell-Derived Prostaglandin E2 Serves as a Request Signal for Macrophage Secretion of Suppressor of Cytokine Signaling 3 during Innate Inflammation.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
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- Expression of the immunomodulator IL-10 in type I pneumocytes of the rat: alterations of IL-10 expression in radiation-induced lung damage.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2007Article
- The myofibroblast: one function, multiple origins.The American journal of pathology · 2007Review
- Lack of evidence for caveolin-1 and CD147 interaction before and after bleomycin-induced lung injury.Histochemistry and cell biology · 2006Article
- Colocalization between caveolin isoforms in the intestinal smooth muscle and interstitial cells of Cajal of the Cav1(+/+) and Cav1 (-/-) mouse.Histochemistry and cell biology · 2006Article
- In situ assessment of oxidant and nitrogenic stress in bleomycin pulmonary fibrosis.Histochemistry and cell biology · 2006Article
- Relevance of the stroma and epithelial-mesenchymal transition (EMT) for the rheumatic diseases.Arthritis research & therapy · 2006Review
- Recent progress in histochemistry and cell biology: the state of the art 2005.Histochemistry and cell biology · 2005Review
- The role of autophagy in idiopathic pulmonary fibrosis: from mechanisms to therapies.Therapeutic advances in respiratory diseaseReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two alveolar epithelial cell lines R3/1 and L2 were screened by immunocytochemical and RT-PCR analysis of epithelial and mesenchymal/contractile marker proteins. R3/1 and L2 cells were tested for their sensitivity to bleomycin (BLM), an anticancer drug, which is proposed to induce changes in lung cell differentiation. Both epithelial cell lines exhibited a mixed phenotype consisting of epithelial (E-cadherin, aquaporin-5 and cytokeratin 8) and myofibroblast-like (vimentin, alpha-SMA and caveolin-3) properties suggesting that the cell lines are arrested in vitro at a certain developmental stage during epithelial-mesenchymal transition (EMT). BLM treatment of R3/1 cells resulted in a partial reversal of this process modifying the cells in an epithelial direction, e.g., upregulation of E-cadherin, aquaporin-5 and other lung epithelial antigens at the mRNA and protein level. L2 cells showed similar alterations following BLM exposure.Immunohistochemical investigation of lung tissue from two different animal models of BLM-induced fibrosis (mouse and rat), revealed no signs of EMT, e.g., myofibroblastic differentiation of alveolar epithelial cells in situ. Immunohistological analysis of tissue samples of the rat model showed a heterogeneous population of myofibroblasts (alpha-SMA+/caveolin-3+, alpha-SMA-/caveolin-3+, and alpha-SMA+/caveolin-3-). These results suggest that BLM, on one hand, induces fibrosis and on the other hand possibly suppresses EMT during fibrogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.