ArticleProceedings of the National Academy of Sciences of the United States of America2005
Human airway epithelial cell determinants of survival and functional phenotype for primary human mast cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 56 citations in OpenAlex.
- Epithelial-Immune Cell Crosstalk in Salivary Gland Tumors: Implications for Tumor Progression and Diagnostic Assessment.International journal of molecular sciences · 2025Article
- Human intraepithelial mast cell differentiation and effector function are directed by TGF-β signaling.The Journal of clinical investigation · 2025Article
- Review
- Control of myeloid cell functions by nociceptors.Frontiers in immunology · 2023Review
- From 2D to 3D Co-Culture Systems: A Review of Co-Culture Models to Study the Neural Cells Interaction.International journal of molecular sciences · 2022Review
- Differential mast cell numbers and characteristics in human tuberculosis pulmonary lesions.Scientific reports · 2021Article
- Human airway mast cells proliferate and acquire distinct inflammation-driven phenotypes during type 2 inflammation.Science immunology · 2021Observational
- Rare Pulmonary Connective Tissue Type Mast Cells Regulate Lung Endothelial Cell Angiogenesis.The American journal of pathology · 2020Article
- Lung Function in African American Children with Asthma Is Associated with Novel Regulatory Variants of the KIT LigandGenetics · 2020Article
- Extracellular vesicles from mast cells induce mesenchymal transition in airway epithelial cells.Respiratory research · 2020Article
- Mast cells in airway diseases and interstitial lung disease.European journal of pharmacology · 2016Review
- Limited replication of influenza A virus in human mast cells.Immunologic research · 2013Article
- Activity of the leukotriene pathway in Barrett's metaplasia and oesophageal adenocarcinoma.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2012Article
- Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps.The Journal of allergy and clinical immunology · 2012Article
- Primary human airway epithelial cell-dependent inhibition of human lung mast cell degranulation.PloS one · 2012Article
- Increased mast cell density and airway responses to allergic and non-allergic stimuli in a sheep model of chronic asthma.PloS one · 2012Article
- Mast cell phenotype, location, and activation in severe asthma. Data from the Severe Asthma Research Program.American journal of respiratory and critical care medicine · 2011Article
- Accumulation of intraepithelial mast cells with a unique protease phenotype in T(H)2-high asthma.The Journal of allergy and clinical immunology · 2010Article
- Human airway smooth muscle promotes human lung mast cell survival, proliferation, and constitutive activation: cooperative roles for CADM1, stem cell factor, and IL-6.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Mast cells (MCs) are found in increased numbers at airway mucosal surfaces in asthmatic patients. Because human airway epithelial cells (HAECs) actively participate in airway inflammatory responses and are in direct contact with MCs in the mucosa, we hypothesized that HAEC-MC interactions may contribute to the differentiation and survival of MCs in the airway mucosa. Here, we show that HAECs express mRNA and protein for soluble and membrane-bound stem cell factor, releasing soluble stem cell factor into the cell culture supernatant at a concentration of 5.9 +/- 0.1 ng per 10(6) HAEC. HAECs were able to support MC survival in coculture in the absence of any exogenous cytokines for at least 4 d. Before the initiation of coculture, MCs were uniformly tryptase and chymase (MC(TC)) double positive, but by 2 d of coculture the majority of MCs expressed tryptase (MC(T)) alone. MCs supported in coculture generated low amounts of cysteinyl-leukotrienes (cys-LT) after FcepsilonRI-dependent activation (0.2 +/- 0.1 ng of cys-LT per 10(6) cells) and required priming with IL-4 and IL-3 during coculture to achieve a quantity of cys-LT generation within the range expected for human lung mucosal MC (26.5 +/- 16 ng of cys-LT per 10(6) cells). In these culture conditions, HAECs were able to direct mucosal MC protease phenotype, but T cell-derived Th2 cytokines were required for the expression of a functional airway MC eicosanoid phenotype. Thus, distinct cell types may direct unique aspects of reactive mucosal MC phenotype in the airways.
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Registered trials
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