ArticleThe Journal of experimental medicine2005
Conditional deletion reveals a cell-autonomous requirement of SLP-76 for thymocyte selection.
Article in The Journal of experimental medicine, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 49 citations in OpenAlex.
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- Bioinformatics analysis of the key genes in osteosarcoma metastasis and immune invasion.Translational pediatrics · 2022Article
- Lymphocyte cytosolic protein 2 is a novel prognostic marker in lung adenocarcinoma.The Journal of international medical research · 2021Article
- Bridging the Gap: Modulatory Roles of the Grb2-Family Adaptor, Gads, in Cellular and Allergic Immune Responses.Frontiers in immunology · 2019Review
- Fit αβ T-cell receptor suppresses leukemogenesis of Pten-deficient thymocytes.Haematologica · 2018Article
- Novel Molecular Insights into Classical and Alternative Activation States of Microglia as Revealed by Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)-based Proteomics.Molecular & cellular proteomics : MCP · 2015Article
- FADD regulates thymocyte development at the β-selection checkpoint by modulating Notch signaling.Cell death & disease · 2014Article
- iNKT cell cytotoxic responses control T-lymphoma growth in vitro and in vivo .Cancer immunology research · 2014Article
- Ras and extracellular signal-regulated kinase signaling in thymocytes and T cells.Trends in immunology · 2013Review
- Murine natural killer immunoreceptors use distinct proximal signaling complexes to direct cell function.Blood · 2013Article
- SLP-76 is required for optimal CXCR4-stimulated T lymphocyte firm arrest to ICAM-1 under shear flow.European journal of immunology · 2012Article
- Deconstructing Ras signaling in the thymus.Molecular and cellular biology · 2012Article
- Cutting edge: IL-2 signals determine the degree of TCR signaling necessary to support regulatory T cell proliferation in vivo.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Physiological and pathophysiological functions of SOCE in the immune system.Frontiers in bioscience (Elite edition) · 2012Review
- The SLP-76 Src homology 2 domain is required for T cell development and activation.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Conditional deletion of SLP-76 in mature T cells abrogates peripheral immune responses.European journal of immunology · 2011Article
- T-cell receptor signals direct the composition and function of the memory CD8+ T-cell pool.Blood · 2010Article
- Loss of tonic T-cell receptor signals alters the generation but not the persistence of CD8+ memory T cells.Blood · 2010Article
- Understanding signal integration through targeted mutations of an adapter protein.FEBS letters · 2010Article
- Independent and cooperative roles of adaptor molecules in proximal signaling during FcepsilonRI-mediated mast cell activation.Molecular and cellular biology · 2010Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The SH2 domain containing leukocyte phosphoprotein of 76 kD (SLP-76) is critical for pre-TCR-mediated maturation to the CD4+CD8+ double positive (DP) stage in the thymus. The absolute block in SLP-76null mice at the CD4-CD8-CD44-CD25+ (double-negative 3, DN3) stage has hindered our understanding of the role of this adaptor in alphabeta TCR-mediated signal transduction in primary thymocytes and peripheral T lymphocytes. To evaluate the requirements for SLP-76 in these events, we used a cre-loxP approach to generate mice that conditionally delete SLP-76 after the DN3 checkpoint. These mice develop DP thymocytes that express the alphabeta TCR on the surface, but lack SLP-76 at the genomic DNA and protein levels. The DP compartment has reduced cellularity in young mice and fails to undergo positive selection to CD4+ or CD8+ single positive (SP) cells in vivo or activation-induced cell death in vitro. A small number of CD4+SP thymocytes are generated, but these cells fail to flux calcium in response to an alphabeta TCR-generated signal. Peripheral T cells are reduced in number, lack SLP-76 protein, and have an abnormal surface phenotype. These studies show for the first time that SLP-76 is required for signal transduction through the mature alphabeta TCR in primary cells of the T lineage.
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