ArticleCell stress & chaperones2005
Differential expression of alphaB-crystallin and Hsp27-1 in anaplastic thyroid carcinomas because of tumor-specific alphaB-crystallin gene (CRYAB) silencing.
Article in Cell stress & chaperones, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Machine learning-based investigation of the cancer protein secretory pathway.PLoS computational biology · 2021Article
- AlphaB-crystallin and breast cancer: role and possible therapeutic strategies.Cell stress & chaperones · 2021Review
- Small Heat Shock Proteins in Cancers: Functions and Therapeutic Potential for Cancer Therapy.International journal of molecular sciences · 2020Review
- The multifaceted nature of αB-crystallin.Cell stress & chaperones · 2020Review
- Progression of the role of CRYAB in signaling pathways and cancers.OncoTargets and therapy · 2019Review
- αB-crystallin: Portrait of a malignant chaperone as a cancer therapeutic target.Pharmacology & therapeutics · 2016Review
- Comparison of microarray expression profiles between follicular variant of papillary thyroid carcinomas and follicular adenomas of the thyroid.BMC genomics · 2015Article
- HspB1, HspB5 and HspB4 in Human Cancers: Potent Oncogenic Role of Some of Their Client Proteins.Cancers · 2014Article
- Expression of the molecular chaperone αB-crystallin in infiltrating ductal breast carcinomas and the significance thereof: an immunohistochemical and proteomics-based strategy.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2012Article
- Promoter methylation of heat shock protein B2 in human esophageal squamous cell carcinoma.International journal of oncology · 2011Article
- alphaB-crystallin is mutant B-RAF regulated and contributes to cyclin D1 turnover in melanocytic cells.Pigment cell & melanoma research · 2010Article
- Expression of heat shock protein 27 and alpha-crystallins in human retinoblastoma after chemoreduction.The British journal of ophthalmology · 2009Article
- Anaplastic thyroid cancer: molecular pathogenesis and emerging therapies.Endocrine-related cancer · 2009Review
- Expression of alpha-crystallins in human sebaceous carcinoma of the eyelid.European journal of ophthalmologyArticle
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Authors and funding
9 authors at 1 institution in 1 country.
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Abstract
Expression of the small heat shock protein alphaB-crystallin in differentiated thyroid tumors has been described recently. In this study, we investigated the molecular mechanisms that affect the expression of alphaB-crystallin in benign goiters (n = 7) and highly malignant anaplastic thyroid carcinomas (ATCs) (n = 3). AlphaB-crystallin expression was compared with that of Hsp27-1. Immunoblot and quantitative real-time (RT) polymerase chain reaction revealed marked downregulation of alphaB-crystallin in all the tested ATCs and the ATC-derived cell line C-643 . In contrast, considerable expression of Hsp27-1 in benign and malignant thyroid tissue was demonstrated. Immunofluorescence analysis revealed no relevant topological differences between benign and malignant thyrocytes in the cytoplasmic staining of both proteins. Consistent and marked downregulation of TFCP2L1 was identified as one of the main mechanisms contributing to CRYAB gene silencing in ATCs. In addition, CRYAB gene promoter methylation seems to occur in distinct ATCs. In silico analysis revealed that the differential expression of alphaB-crystallin and Hsp27-1 results from differences between the alphaB-crystallin and Hsp27-1 promoter fragments (712 bp upstream from the transcriptional start site). Biological activity of the analyzed promoter element is confirmed by its heat shock inducibility. In conclusion, we demonstrate downregulation of alphaB-crystallin expression in highly dedifferentiated ATCs because of a tumor-specific transcription factor pattern. The differential expression of alphaB-crystallin and Hsp27-1 indicates functional differences between both proteins.
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