ArticleEuropean biophysics journal : EBJ2006
Probing the interaction between vesicular stomatitis virus and phosphatidylserine.
Article in European biophysics journal : EBJ, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 52 citations in OpenAlex.
- Probing living cell dynamics and molecular interactions using atomic force microscopy.Biophysical reviews · 2024Review
- Insights into Polyphenol-Lipid Interactions: Chemical Methods, Molecular Aspects and Their Effects on Membrane Structures.Plants (Basel, Switzerland) · 2022Review
- Interactions between Hydrolysable Tannins and Lipid Vesicles fromMolecules (Basel, Switzerland) · 2022Article
- Acquired chemoresistance can lead to increased resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus.Molecular therapy oncolytics · 2022Article
- NPC1-regulated dynamic of clathrin-coated pits is essential for viral entry.Science China. Life sciences · 2022Article
- Characterization of the Impact of Oncolytic Vesicular Stomatitis Virus on the Trafficking, Phenotype, and Antigen Presentation Potential of Neutrophils and Their Ability to Acquire a Non-Structural Viral Protein.International journal of molecular sciences · 2020Article
- Experimental Evolution Generates Novel Oncolytic Vesicular Stomatitis Viruses with Improved Replication in Virus-Resistant Pancreatic Cancer Cells.Journal of virology · 2020Article
- Cell Cycle Arrest in GJournal of virology · 2019Article
- Perturbation of Intracellular Cholesterol and Fatty Acid Homeostasis During Flavivirus Infections.Frontiers in immunology · 2018Review
- Ruxolitinib and Polycation Combination Treatment Overcomes Multiple Mechanisms of Resistance of Pancreatic Cancer Cells to Oncolytic Vesicular Stomatitis Virus.Journal of virology · 2017Article
- Multi-virion infectious units arise from free viral particles in an enveloped virus.Nature microbiology · 2017Article
- Article
- Lipid interactions during virus entry and infection.Cellular microbiology · 2014Review
- Understanding and altering cell tropism of vesicular stomatitis virus.Virus research · 2013Review
- Entry of rhabdoviruses into animal cells.Advances in experimental medicine and biology · 2013Review
- Role of lipids in virus replication.Cold Spring Harbor perspectives in biology · 2011Review
- Cell entry of enveloped viruses.Advances in genetics · 2011Review
- Pepscan mapping of viral hemorrhagic septicemia virus glycoprotein G major lineal determinants implicated in triggering host cell antiviral responses mediated by type I interferon.Journal of virology · 2010Article
- Lentiviral vectors for immune cells targeting.Immunopharmacology and immunotoxicology · 2010Review
- Endoplasmic reticulum chaperone gp96 is essential for infection with vesicular stomatitis virus.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
- Erratum issuedPoian, Andrea T Da [corrected to Da Poian, Andrea T]
Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The entry of enveloped animal viruses into their host cells always depends on membrane fusion triggered by conformational changes in viral envelope glycoproteins. Vesicular stomatitis virus (VSV) infection is mediated by virus spike glycoprotein G, which induces membrane fusion between the viral envelope and the endosomal membrane at the acidic environment of this compartment. In this work, we evaluated VSV interactions with membranes of different phospholipid compositions, at neutral and acidic pH, using atomic force microscopy (AFM) operating in the force spectroscopy mode, isothermal calorimetry (ITC) and molecular dynamics simulation. We found that the binding forces differed dramatically depending on the membrane phospholipid composition, revealing a high specificity of G protein binding to membranes containing phosphatidylserine (PS). In a previous work, we showed that the sequence corresponding amino acid 164 of VSV G protein was as efficient as the virus in catalyzing membrane fusion at pH 6.0. Here, we used this sequence to explore VSV-PS interaction using ITC. We found that peptide binding to membranes was exothermic, suggesting the participation of electrostatic interactions. Peptide-membrane interaction at pH 7.5 was shown to be specific to PS and dependent on the presence of His residues in the fusion peptide. The application of the simplified continuum Gouy-Chapman theory to our system predicted a pH of 5.0 at membrane surface, suggesting that the His residues should be protonated when located close to the membrane. Molecular dynamics simulations suggested that the peptide interacts with the lipid bilayer through its N-terminal residues, especially Val(145) and His(148).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.