ArticleGenetics2005
Dissection of Arp2/3 complex actin nucleation mechanism and distinct roles for its nucleation-promoting factors in Saccharomyces cerevisiae.
Article in Genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Bsp1, a fungal CPI motif protein, regulates actin filament capping in endocytosis and cytokinesis.Molecular biology of the cell · 2024Article
- Spatio-temporal regulation of endocytic protein assembly by SH3 domains in yeast.Molecular biology of the cell · 2023Article
- A Flow Cytometry-Based Phenotypic Screen To Identify Novel Endocytic Factors inG3 (Bethesda, Md.) · 2018Article
- Identification of an ATP-controlled allosteric switch that controls actin filament nucleation by Arp2/3 complex.Nature communications · 2016Article
- Article
- Actin and endocytosis in budding yeast.Genetics · 2015Review
- Article
- Insertions within the actin core of actin-related protein 3 (Arp3) modulate branching nucleation by Arp2/3 complex.The Journal of biological chemistry · 2013Article
- Lsb1 is a negative regulator of las17 dependent actin polymerization involved in endocytosis.PloS one · 2013Article
- Coronin is a component of the endocytic collar of hyphae of Neurospora crassa and is necessary for normal growth and morphogenesis.PloS one · 2012Article
- Mechanism of a concentration-dependent switch between activation and inhibition of Arp2/3 complex by coronin.The Journal of biological chemistry · 2011Article
- The p40/ARPC1 subunit of Arp2/3 complex performs multiple essential roles in WASp-regulated actin nucleation.The Journal of biological chemistry · 2010Article
- The F-BAR protein Syp1 negatively regulates WASp-Arp2/3 complex activity during endocytic patch formation.Current biology : CB · 2009Article
- Polarized growth in the absence of F-actin in Saccharomyces cerevisiae exiting quiescence.PloS one · 2008Article
- btn1 affects endocytosis, polarization of sterol-rich membrane domains and polarized growth in Schizosaccharomyces pombe.Traffic (Copenhagen, Denmark) · 2008Article
- Arp2 links autophagic machinery with the actin cytoskeleton.Molecular biology of the cell · 2008Article
- Distinct roles for Arp2/3 regulators in actin assembly and endocytosis.PLoS biology · 2008Article
- Interaction of the endocytic scaffold protein Pan1 with the type I myosins contributes to the late stages of endocytosis.Molecular biology of the cell · 2007Article
- Insights into the influence of nucleotides on actin family proteins from seven structures of Arp2/3 complex.Molecular cell · 2007Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Actin nucleation by the Arp2/3 complex is under tight control, remaining inactive until stimulation by nucleation-promoting factors (NPFs). Although multiple NPFs are expressed in most cell types, little is known about how they are coordinated and whether they perform similar or distinct functions. We examined genetic relationships among the four S. cerevisiae NPFs. Combining las17delta with pan1-101 or myo3delta myo5delta was lethal at all temperatures, whereas combining pan1-101 with myo3delta myo5delta showed no genetic interaction and abp1delta partially suppressed las17delta. These data suggest that NPFs have distinct and overlapping functions in vivo. We also tested genetic interactions between each NPF mutant and seven different temperature-sensitive arp2 alleles and purified mutant Arp2/3 complexes to compare their activities. Two arp2 alleles with mutations at the barbed end were severely impaired in nucleation, providing the first experimental evidence that Arp2 nucleates actin at its barbed end in vitro and in vivo. Another arp2 allele caused partially unregulated ("leaky") nucleation in the absence of NPFs. Combining this mutant with a partially unregulated allele in a different subunit of Arp2/3 complex was lethal, suggesting that cells cannot tolerate high levels of unregulated activity. Genetic interactions between arp2 alleles and NPF mutants point to Abp1 having an antagonistic role with respect to other NPFs, possibly serving to attenuate their stronger activities. In support of this model, Abp1 binds strongly to Arp2/3 complex, yet has notably weak nucleation-promoting activity and inhibits Las17 activity on Arp2/3 complex in a dose-responsive manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.