ArticleBiophysical journal2005
Influence of hydrocortisone on the mechanical properties of the cerebral endothelium in vitro.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 54 citations in OpenAlex.
- TheMaterials today. Bio · 2026Review
- Dual-modified Liposomes Encapsulating Nucleic Acids (pApoE2 or pGFP) for Transport Studies Across a Hydrocortisone-enhanced In Vitro Blood-brain Barrier Model for CNS Therapeutic Screening.Pharmaceutical research · 2025Article
- Vascularizing the brainiScience · 2022Review
- Article
- Optimization of an oral mucosaTissue barriers · 2020Article
- Controversial roles for dexamethasone in glioblastoma - Opportunities for novel vascular targeting therapies.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2019Review
- Perspective: The role of mechanobiology in the etiology of brain metastasis.APL bioengineering · 2018Article
- The mechanical properties of individual cell spheroids.Scientific reports · 2017Article
- An In Vitro Model of the Blood-brain Barrier Using Impedance Spectroscopy: A Focus on T Cell-endothelial Cell Interaction.Journal of visualized experiments : JoVE · 2016Article
- Article
- ARP2/3-mediated junction-associated lamellipodia control VE-cadherin-based cell junction dynamics and maintain monolayer integrity.Molecular biology of the cell · 2014Article
- Modelling the endothelial blood-CNS barriers: a method for the production of robust in vitro models of the rat blood-brain barrier and blood-spinal cord barrier.BMC neuroscience · 2013Article
- Mechanical responsiveness of the endothelial cell of Schlemm's canal: scope, variability and its potential role in controlling aqueous humour outflow.Journal of the Royal Society, Interface · 2012Article
- Depth-sensing analysis of cytoskeleton organization based on AFM data.European biophysics journal : EBJ · 2012Article
- Young's modulus of elasticity of Schlemm's canal endothelial cells.Biomechanics and modeling in mechanobiology · 2010Article
- Elasticity measurement of living cells with an atomic force microscope: data acquisition and processing.Pflugers Archiv : European journal of physiology · 2008Article
- Probing nanomechanical properties from biomolecules to living cells.Pflugers Archiv : European journal of physiology · 2008Review
- Elastic properties of the cell surface and trafficking of single AMPA receptors in living hippocampal neurons.Biophysical journal · 2007Article
- In vitro and ex vivo models of the oral mucosa as platforms for the validation of novel drug delivery systems.Journal of tissue engineeringReview
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cerebral endothelial cells accomplish the barrier functions between blood and brain interstitium. Structural features are the tight junctions between adjacent endothelial cells and the formation of marginal folds at the cell-cell contacts. The glucocorticoid hydrocortisone (HC) has been reported to enforce the blood-brain-barrier in vitro measurable by an increase of the transendothelial electrical resistance. This study shows the impact of HC on the mechanical and morphological properties of confluent cell layers of brain microvascular endothelial cells. HC induces an increase in height of these marginal folds and a reduction of the intercellular contact surface. These morphological changes are accompanied by changes in cell elasticity. Staining of fibrous actin indicates that HC induces a reorganization of the actin cortex. The quantitative determination of the local elastic properties of cells reveals for the first time an HC-induced increase of the representative Young's modulus according to cytoskeletal rearrangements. For this study, cells of two different species, porcine brain capillary endothelial cells and murine brain capillary endothelial cells, were used yielding similar results, which clearly demonstrates that the HC effect on the cell elasticity is species independent.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.