ArticleProceedings of the National Academy of Sciences of the United States of America2005
Partner-regulated interaction of IFN regulatory factor 8 with chromatin visualized in live macrophages.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 48 citations in OpenAlex.
- Cytoplasmic Shift of Interferon Regulatory Factors Co-Evolved With Jawed Vertebrate Innate Immunity.Journal of medical virology · 2025Article
- Article
- Review
- Brd4 regulates NLRC4 inflammasome activation by facilitating IRF8-mediated transcription of Naips.The Journal of cell biology · 2021Article
- A general computational approach to predicting synergistic transcriptional cores that determine cell subpopulation identities.Nucleic acids research · 2019Article
- Pivotal role of innate myeloid cells in cerebral post-ischemic sterile inflammation.Seminars in immunopathology · 2018Review
- Total cellular protein presence of the transcription factor IRF8 does not necessarily correlate with its nuclear presence.Methods (San Diego, Calif.) · 2017Article
- MMP3-mediated tumor progression is controlled transcriptionally by a novel IRF8-MMP3 interaction.Oncotarget · 2015Article
- Transcription factor IRF8 plays a critical role in the development of murine basophils and mast cells.Blood · 2015Article
- A reporter mouse reveals lineage-specific and heterogeneous expression of IRF8 during lymphoid and myeloid cell differentiation.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- The transcription factor IRF8 activates integrin-mediated TGF-β signaling and promotes neuroinflammation.Immunity · 2014Article
- Interferon-regulatory factors determine macrophage phenotype polarization.Mediators of inflammation · 2013Review
- The small ubiquitin-like modifier-deconjugating enzyme sentrin-specific peptidase 1 switches IFN regulatory factor 8 from a repressor to an activator during macrophage activation.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Contribution of a TANK-binding kinase 1-interferon (IFN) regulatory factor 7 pathway to IFN-γ-induced gene expression.Molecular and cellular biology · 2012Article
- Discovering causal signaling pathways through gene-expression patterns.Nucleic acids research · 2010Article
- Regulation of immunity and oncogenesis by the IRF transcription factor family.Cancer immunology, immunotherapy : CII · 2010Review
- Heterodimerization with different Jun proteins controls c-Fos intranuclear dynamics and distribution.The Journal of biological chemistry · 2010Article
- Visualization of molecular interactions using bimolecular fluorescence complementation analysis: characteristics of protein fragment complementation.Chemical Society reviews · 2009Review
- Differentiation driven changes in the dynamic organization of Basal transcription initiation.PLoS biology · 2009Article
- Cholera toxin inhibits IL-12 production and CD8alpha+ dendritic cell differentiation by cAMP-mediated inhibition of IRF8 function.The Journal of experimental medicine · 2009Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
IFN regulatory factor (IRF) 8 is a transcription factor that directs macrophage differentiation. By fluorescence recovery after photobleaching, we visualized the movement of IRF8-GFP in differentiating macrophages. Recovery data fitted to mathematical models revealed two binding states for IRF8. The majority of IRF8 was highly mobile and transiently interacted with chromatin, whereas a small fraction of IRF8 bound to chromatin more stably. IRF8 mutants that did not stimulate macrophage differentiation showed a faster recovery, revealing little interaction with chromatin. A macrophage activation signal by IFN-gamma/LPS led to a global slowdown of IRF8 movement, leading to increased chromatin binding. In fibroblasts where IRF8 has no known function, WT IRF8 moved as fast as the mutants, indicating that IRF8 does not interact with chromatin in these cells. However, upon introduction of IRF8 binding partners, PU.1 and/or IRF1, the mobility of IRF8 was markedly reduced, producing a more stably bound component. Together, IRF8-chromatin interaction is dynamic in live macrophages and influenced by partner proteins and immunological stimuli.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.