ArticleJournal of virological methods2006
Improved detection of bovine coronavirus N gene in faeces of calves infected naturally by a semi-nested PCR assay and an internal control.
Article in Journal of virological methods, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Genetic diversity of bovine coronaviruses from recurrent outbreaks on a large-scale cattle farm.Tropical animal health and production · 2026Article
- Evaluating the presence of bovine coronavirus in diarrheic calves in Lahore, Punjab, Pakistan: a food security risk.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Prevalence and genetic characterization of diarrhea viruses among cattle in Guangdong, China.BMC veterinary research · 2025Article
- Molecular detection of bovine coronavirus in the environment of a naturally infected dairy cattle herd.Tropical animal health and production · 2025Article
- Bovine Coronavirus in diarrheic pre-weaned calves in Egypt: prevalence, risk factors, and the associated biochemical alterations.Tropical animal health and production · 2025Article
- Molecular detection of coronavirus in camelids and bovines using real-time quantitative polymerase chain reaction in Wasit Province, Iraq.Open veterinary journal · 2025Article
- Establishment of an indirect ELISA method for detecting bovine coronavirus antibodies based on N protein.Frontiers in veterinary science · 2025Article
- Comparative Insights into Acute Gastroenteritis in Cattle Caused by Bovine Rotavirus A and Bovine Coronavirus.Veterinary sciences · 2024Review
- Respiratory illness in young and adult cattle caused by bovine viral diarrhea virus subgenotype 2b in singular and mixed bacterial infection in a BVDV-vaccinated dairy herd.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Microbiological Profile of the Upper and Lower Respiratory Tract of Suckling and Weaned Dairy Calves with Acute Respiratory Disease.Veterinary sciences · 2024Article
- Development of the Intestinal Microbiota of Dairy Calves and Changes Associated withMicroorganisms · 2024Article
- Advances in Laboratory Diagnosis of Coronavirus Infections in Cattle.Pathogens (Basel, Switzerland) · 2024Review
- A comprehensive molecular analysis of bovine coronavirus strains isolated from Brazil and comparison of a wild-type and cell culture-adapted strain associated with respiratory disease.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- The Role ofPathogens (Basel, Switzerland) · 2024Article
- Screening of Bovine Coronavirus Multiepitope Vaccine Candidates: An Immunoinformatics Approach.Transboundary and emerging diseases · 2024Article
- Specific and sensitive detection of bovine coronavirus using CRISPR-Cas13a combined with RT-RAA technology.Frontiers in veterinary science · 2024Article
- Neglected bacterial infections associated to bovine respiratory disease in lactating cows from high-yielding dairy cattle herds.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Histophilus somni disease conditions with simultaneous infections by ovine gammaherpesvirus 2 in cattle herds from Southern Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Association of ovine gammaherpesvirus 2 with an outbreak of acute respiratory disease in dairy cattle.Scientific reports · 2023Article
- Possible Association of Bovine Gammaherpesvirus 6 with Pulmonary Disease in a Cow.Animals : an open access journal from MDPI · 2023Article
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4 authors.
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Abstract
Bovine coronavirus (BCoV), a positive sense single-stranded RNA virus, is an important causative agent of neonatal diarrhoea in calves from beef and dairy cattle worldwide. The routine detection and diagnosis of BCoV have been mainly dependent on assays with low sensitivity. The aim of the present study was to develop and evaluate a semi-nested PCR (SN-PCR) to amplify a 251bp fragment of BCoV N gene from fresh (n=25) and frozen (n=25) diarrhoeic faecal samples of naturally infected calves. To improve detection of BCoV in faecal samples by the SN-PCR an internal control was developed, and the results were compared with a conventional RT-PCR assay. The rates of positive samples by SN-PCR and RT-PCR were 24% (12/50) and 8% (4/50), respectively (K=0.43). Only fresh samples were positive in RT-PCR while the SN-PCR detected BCoV in both fresh and frozen faecal samples. The sensitivity of SN-PCR was determined by 10-fold serial dilutions of the BCoV Kakegawa strain (HA titre: 256) that was detected until 10(-7) dilution. The specificity of the amplicons was assessed by restriction fragment length polymorphism and sequence analysis. The inclusion of an internal control provides a way to detect assay inhibition in faecal samples and failure of nucleic acid extraction that allow reduction of the number of false-negative results.
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