ArticleEMBO reports2005
Developmental regulation of a proinsulin messenger RNA generated by intron retention.
Article in EMBO reports, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 46 citations in OpenAlex.
- Retained introns in long RNA-seq reads are not reliably detected in sample-matched short reads.Genome biology · 2022Article
- Article
- Genome-wide analysis of alternative splicing during human heart development.Scientific reports · 2016Article
- Non-coding functions of alternative pre-mRNA splicing in development.Seminars in cell & developmental biology · 2015Review
- Transcriptome analysis of human tissues and cell lines reveals one dominant transcript per gene.Genome biology · 2013Article
- Subcellular RNA sequencing reveals broad presence of cytoplasmic intron-sequence retaining transcripts in mouse and rat neurons.PloS one · 2013Article
- Selected imprinting of INS in the marsupial.Epigenetics & chromatin · 2012Article
- Coordinated regulation of neuronal mRNA steady-state levels through developmentally controlled intron retention.Genes & development · 2012Article
- Proinsulin: from hormonal precursor to neuroprotective factor.Frontiers in molecular neuroscience · 2011Article
- Allele-specific recognition of the 3' splice site of INS intron 1.Human genetics · 2010Article
- Medicago truncatula contains a second gene encoding a plastid located glutamine synthetase exclusively expressed in developing seeds.BMC plant biology · 2010Article
- Unconstrained mining of transcript data reveals increased alternative splicing complexity in the human transcriptome.Nucleic acids research · 2010Article
- Cardiogenesis: an embryological perspective.Journal of cardiovascular translational research · 2010Review
- The 5' leader of the mRNA encoding the marek's disease virus serotype 1 pp14 protein contains an intronic internal ribosome entry site with allosteric properties.Journal of virology · 2009Article
- Identification of myo-inositol-3-phosphate synthase isoforms: characterization, expression, and putative role of a 16-kDa gamma(c) isoform.The Journal of biological chemistry · 2009Article
- Oxytocin and vasopressin gene expression and RNA splicing patterns in the rat supraoptic nucleus.Physiological genomics · 2008Article
- Hormonal regulation of cardiac KCNE2 gene expression.Molecular and cellular endocrinology · 2008Article
- Comparative analysis of sequence features involved in the recognition of tandem splice sites.BMC genomics · 2008Article
- Restricted expression of Epstein-Barr virus latent genes in murine B cells derived from embryonic stem cells.PloS one · 2008Article
- Intron-3 retention/splicing controls neuronal expression of apolipoprotein E in the CNS.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proinsulin gene expression regulation and function during early embryonic development differ remarkably from those found in postnatal organisms. The embryonic proinsulin protein content decreased from gastrulation to neurulation in contrast with the overall proinsulin messenger RNA increase. This is due to increasing levels of a proinsulin mRNA variant generated by intron 1 retention in the 5' untranslated region. Inclusion of intron 1 inhibited proinsulin translation almost completely without affecting nuclear export or cytoplasmic decay. The novel proinsulin mRNA isoform expression was developmentally regulated and tissue specific. The proportion of intron retention increased from gastrulation to organogenesis, was highest in the heart tube and presomitic region, and could not be detected in the pancreas. Notably, proinsulin addition induced cardiac marker gene expression in the early embryonic stages when the translationally active transcript was expressed. We propose that regulated unproductive splicing and translation is a mechanism that regulates proinsulin expression in accordance with specific requirements in developing vertebrates.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.