ArticleInfection and immunity2005
Human dendritic cell activity against Histoplasma capsulatum is mediated via phagolysosomal fusion.
Article in Infection and immunity, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Extracellular vesicles from DistinctACS infectious diseases · 2025Article
- Pathogenicity & virulence ofVirulence · 2022Review
- Antifungal activity of dendritic cell lysosomal proteins against Cryptococcus neoformans.Scientific reports · 2021Article
- Histoplasma Capsulatum: Mechanisms for Pathogenesis.Current topics in microbiology and immunology · 2019Review
- Dectin-2 is a primary receptor for NLRP3 inflammasome activation in dendritic cell response to Histoplasma capsulatum.PLoS pathogens · 2017Article
- CD103+ Conventional Dendritic Cells Are Critical for TLR7/9-Dependent Host Defense against Histoplasma capsulatum, an Endemic Fungal Pathogen of Humans.PLoS pathogens · 2016Article
- Intracellular Pathogens within Alveolar Macrophages in a Patient with HIV Infection: Diagnostic Challenge.Infectious disease reports · 2015Article
- Review
- NLRP3 inflammasome activation by Paracoccidioides brasiliensis.PLoS neglected tropical diseases · 2013Article
- Celecoxib improves host defense through prostaglandin inhibition during Histoplasma capsulatum infection.Mediators of inflammation · 2013Article
- Mechanisms of dendritic cell lysosomal killing of Cryptococcus.Scientific reports · 2012Article
- Antigen-presenting dendritic cells rescue CD4-depleted CCR2-/- mice from lethal Histoplasma capsulatum infection.Infection and immunity · 2010Article
- Autophagy supports Candida glabrata survival during phagocytosis.Cellular microbiology · 2010Article
- Mechanism involved in phagocytosis and killing of Listeria monocytogenes by Acanthamoeba polyphaga.Parasitology research · 2009Article
- The CCL7-CCL2-CCR2 axis regulates IL-4 production in lungs and fungal immunity.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Cryptococcus neoformans enters the endolysosomal pathway of dendritic cells and is killed by lysosomal components.Infection and immunity · 2008Article
- Histoplasma capsulatum manifests preferential invasion of phagocytic subpopulations in murine lungs.Journal of leukocyte biology · 2008Article
- A monoclonal antibody to Histoplasma capsulatum alters the intracellular fate of the fungus in murine macrophages.Eukaryotic cell · 2008Article
- TNF-alpha antagonism generates a population of antigen-specific CD4+CD25+ T cells that inhibit protective immunity in murine histoplasmosis.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- Expression of hygromycin phosphotransferase alters virulence of Histoplasma capsulatum.Eukaryotic cell · 2007Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Histoplasma capsulatum is a fungal pathogen that requires the induction of cell-mediated immunity (CMI) for host survival. We have demonstrated that human dendritic cells (DC) phagocytose H. capsulatum yeasts and, unlike human macrophages (Mø) that are permissive for intracellular growth, DC killed and degraded the fungus. In the present study, we sought to determine whether the mechanism(s) by which DC kill Histoplasma is via lysosomal hydrolases, via the production of toxic oxygen metabolites, or both. Phagosome-lysosome fusion (PL-fusion) was quantified by using fluorescein isothiocyanate-dextran and phase and fluorescence microscopy and by electron microscopy with horseradish peroxidase colloidal gold to label lysosomes. Unlike Mphi, Histoplasma-infected DC exhibited marked PL-fusion. The addition of suramin to Histoplasma-infected DC inhibited PL-fusion and DC fungicidal activity. Incubation of Histoplasma-infected DC at 18 degrees C also concomitantly reduced PL-fusion and decreased the capacity of DC to kill and degrade H. capsulatum yeasts. Further, culture of Histoplasma-infected DC in the presence of bafilomycin, an inhibitor of the vacuolar ATPase, did not block DC anti-Histoplasma activity, indicating that phagosome acidification was not required for lysosome enzyme activity. In contrast, culture of Histoplasma-infected DC in the presence of inhibitors of the respiratory burst or inhibitors of NO synthase had little to no effect on DC fungicidal activity. These data suggest that the major mechanism by which human DC mediate anti-Histoplasma activity is through the exposure of yeasts to DC lysosomal hydrolases. Thus, DC can override one of the strategies used by H. capsulatum yeasts to survive intracellularly within Mø.
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