ArticleInfection and immunity2005
Identification of a novel sialic acid transporter in Haemophilus ducreyi.
Article in Infection and immunity, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 42 citations in OpenAlex.
- Structural studies and functional engineering of NanX: an anhydro-sialic acid transporter from Escherichia coli.FEBS open bio · 2026Article
- Expanding salivary biomarker detection by creating a synthetic neuraminic acid sensor via chimeragenesis.Applied and environmental microbiology · 2026Article
- A bidirectionalbioRxiv : the preprint server for biology · 2026Article
- The physiological characteristics and applications of sialic acid.NPJ science of food · 2025Review
- Review
- Bacterial Sialic Acid Catabolism at the Host-Microbe Interface.Journal of microbiology (Seoul, Korea) · 2023Review
- Sialic Acid 4-N-Piperazine and Piperidine Derivatives Bind with High Affinity to the P. mirabilis Sialic Acid Sodium Solute Symporter.ChemMedChem · 2022Article
- Biological functions of sialic acid as a component of bacterial endotoxin.Frontiers in microbiology · 2022Review
- Multiple evolutionary origins reflect the importance of sialic acid transporters in the colonization potential of bacterial pathogens and commensals.Microbial genomics · 2021Article
- Structural and Biosynthetic Diversity of Nonulosonic Acids (NulOs) That Decorate Surface Structures in Bacteria.Trends in microbiology · 2021Review
- The Potential Use of Sialic Acid From Edible Bird's Nest to Attenuate Mitochondrial Dysfunction byFrontiers in pharmacology · 2021Article
- Uncovering a novel molecular mechanism for scavenging sialic acids in bacteria.The Journal of biological chemistry · 2020Article
- Elucidation of a sialic acid metabolism pathway in mucus-foraging Ruminococcus gnavus unravels mechanisms of bacterial adaptation to the gut.Nature microbiology · 2019Article
- Molecular characterization of the interaction of sialic acid with the periplasmic binding protein fromThe Journal of biological chemistry · 2018Article
- Substrate-bound outward-open structure of a NaNature communications · 2018Article
- "Just a spoonful of sugar...": import of sialic acid across bacterial cell membranes.Biophysical reviews · 2018Review
- The Sodium Sialic Acid Symporter FromFrontiers in chemistry · 2018Article
- Article
- Host Sialic Acids: A Delicacy for the Pathogen with Discerning Taste.Microbiology spectrum · 2015Review
- Improving the efficiency of transposon mutagenesis in Salmonella enteritidis by overcoming host-restriction barriers.Molecular biotechnology · 2014Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Haemophilus ducreyi, the causative agent of chancroid, produces a lipooligosaccharide (LOS) which terminates in N-acetyllactosamine. This glycoform can be further extended by the addition of a single sialic acid residue to the terminal galactose moiety. H. ducreyi does not synthesize sialic acid, which must be acquired from the host during infection or from the culture medium when the bacteria are grown in vitro. However, H. ducreyi does not have genes that are highly homologous to the genes encoding known bacterial sialic acid transporters. In this study, we identified the sialic acid transporter by screening strains in a library of random transposon mutants for those mutants that were unable to add sialic acid to N-acetyllactosamine-containing LOS. Mutants that reacted with the monoclonal antibody 3F11, which recognizes the terminal lactosamine structure, and lacked reactivity with the lectin Maackia amurensis agglutinin, which recognizes alpha2,3-linked sialic acid, were further characterized to demonstrate that they produced a N-acetyllactosamine-containing LOS by silver-stained sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometric analyses. The genes interrupted in these mutants were mapped to a four-gene cluster with similarity to genes encoding bacterial ABC transporters. Uptake assays using radiolabeled sialic acid confirmed that the mutants were unable to transport sialic acid. This study is the first report of bacteria using an ABC transporter for sialic acid uptake.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.