ArticleInfection and immunity2005
Activation and mitogen-activated protein kinase regulation of transcription factors Ets and NF-kappaB in Mycobacterium-infected macrophages and role of these factors in tumor necrosis factor alpha and nitric oxide synthase 2 promoter function.
Article in Infection and immunity, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Macrophage Immune Response Suppression by Recombinant Mycobacterium tuberculosis Antigens, the ESAT-6, CFP-10, and ESAT-6/CFP-10 Fusion Proteins.Iranian journal of medical sciences · 2016Article
- Article
- Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response.PloS one · 2014Article
- Leishmania major inhibits IL-12 in macrophages by signalling through CR3 (CD11b/CD18) and down-regulation of ETS-mediated transcription.Parasite immunology · 2013Article
- Mycobacterium tuberculosis PE_PGRS17 promotes the death of host cell and cytokines secretion via Erk kinase accompanying with enhanced survival of recombinant Mycobacterium smegmatis.Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research · 2013Article
- The SRC family tyrosine kinase HCK and the ETS family transcription factors SPIB and EHF regulate transcytosis across a human follicle-associated epithelium model.The Journal of biological chemistry · 2013Article
- Article
- Pioglitazone, PPARγ agonist, attenuates experimental autoimmune neuritis.Inflammation · 2012Article
- Mitogen-activated protein kinases mediate Mycobacterium tuberculosis-induced CD44 surface expression in monocytes.Journal of biosciences · 2012Article
- The non-pathogenic mycobacteria M. smegmatis and M. fortuitum induce rapid host cell apoptosis via a caspase-3 and TNF dependent pathway.BMC microbiology · 2010Article
- Sphingosine kinase-1 (SphK-1) regulates Mycobacterium smegmatis infection in macrophages.PloS one · 2010Article
- Macrophage response to apoptotic cells varies with the apoptotic trigger and is not altered by a deficiency in LRP expression.Journal of innate immunity · 2010Article
- Monocyte/macrophage androgen receptor suppresses cutaneous wound healing in mice by enhancing local TNF-alpha expression.The Journal of clinical investigation · 2009Article
- Transcriptional analysis of ESAT-6 cluster 3 in Mycobacterium smegmatis.BMC microbiology · 2009Article
- Mycobacterium abscessus and M. avium trigger Toll-like receptor 2 and distinct cytokine response in human cells.American journal of respiratory cell and molecular biology · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Previous studies have shown that primary murine macrophages infected with Mycobacterium avium produced lower levels of tumor necrosis factor alpha (TNF-alpha) and inducible nitric oxide synthase 2 (NOS2) compared to cells infected with nonpathogenic Mycobacterium smegmatis. TNF-alpha and NOS2 levels correlated with and were dependent on the activation of mitogen-activated protein kinases (MAPKs) p38 and extracellular signal-regulated kinase 1/2 (ERK1/2). To define the macrophage transcriptional responses dependent on ERK1/2 activation following a mycobacterial infection, we used RAW 264.7 cells transfected with a TNF-alpha or NOS2 promoter vector. We determined that macrophages infected with M. avium compared to M. smegmatis showed diminished TNF-alpha and NOS2 promoter activity. A more pronounced difference in promoter activity was observed when only the consensus ETS and NF-kappaB binding sites were used as promoters. Mutational analysis of the ETS and NF-kappaB binding sites present on the TNF-alpha and NOS2 promoters, respectively, showed that these sites were essential for a functional promoter. Moreover, the Ets/Elk but not the NF-kappaB transcriptional response was dependent on ERK1/2. This correlated with the requirement for ERK1/2 in TNF-alpha but not NOS2 promoter activity. Our data indicate that the increased Ets/Elk and NF-kappaB promoter activities associated with M. smegmatis-infected macrophages are responsible, at least in part, for the increased TNF-alpha and NOS2 production observed in these infected cells and that ERK1/2 is required for Ets/Elk activity and full TNF-alpha production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.