ArticleInfection and immunity2005
Adherence to human vaginal epithelial cells signals for increased expression of Trichomonas vaginalis genes.
Article in Infection and immunity, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 63 citations in OpenAlex.
- Analysis ofMicroorganisms · 2026Article
- The interaction between adhesion protein 33 (TvAP33) and BNIP3 mediates the adhesion and pathogenicity of Trichomonas vaginalis to host cells.Parasites & vectors · 2023Article
- RNA-Binding Proteins in Trichomonas vaginalis: Atypical Multifunctional Proteins.Biomolecules · 2015Review
- Iron from haemoglobin and haemin modulates nucleotide hydrolysis in Trichomonas vaginalis.Memorias do Instituto Oswaldo Cruz · 2015Article
- The Pathogenesis of Human Cervical Epithelium Cells Induced by Interacting with Trichomonas vaginalis.PloS one · 2015Article
- α -Actinin TvACTN3 of Trichomonas vaginalis is an RNA-binding protein that could participate in its posttranscriptional iron regulatory mechanism.BioMed research international · 2014Article
- Biological roles of cysteine proteinases in the pathogenesis of Trichomonas vaginalis.Parasite (Paris, France) · 2014Review
- Epitopes of the highly immunogenic Trichomonas vaginalis α-actinin are serodiagnostic targets for both women and men.Journal of clinical microbiology · 2013Article
- Prospective study of effect modification by Toll-like receptor 4 variation on the association between Trichomonas vaginalis serostatus and prostate cancer.Cancer causes & control : CCC · 2013Article
- Identification, characterization, and synthesis of peptide epitopes and a recombinant six-epitope protein for Trichomonas vaginalis serodiagnosis.ImmunoTargets and therapy · 2013Article
- Comparative transcriptomic and proteomic analyses of Trichomonas vaginalis following adherence to fibronectin.Infection and immunity · 2012Article
- Regulation of gene expression in protozoa parasites.Journal of biomedicine & biotechnology · 2010Review
- Impact of T. vaginalis infection on innate immune responses and reproductive outcome.Journal of reproductive immunology · 2009Review
- Genetic identity and differential gene expression between Trichomonas vaginalis and Trichomonas tenax.BMC microbiology · 2009Article
- Immunogenic and plasminogen-binding surface-associated alpha-enolase of Trichomonas vaginalis.Infection and immunity · 2008Article
- Characterization of the Trichomonas vaginalis surface-associated AP65 and binding domain interacting with trichomonads and host cells.BMC microbiology · 2007Article
- The proteins secreted by Trichomonas vaginalis and vaginal epithelial cell response to secreted and episomally expressed AP65.Cellular microbiology · 2007Article
- Antisense RNA decreases AP33 gene expression and cytoadherence by T. vaginalis.BMC microbiology · 2007Article
- A novel surface protein of Trichomonas vaginalis is regulated independently by low iron and contact with vaginal epithelial cells.BMC microbiology · 2006Article
- Mechanisms of Tritrichomonas foetus Pathogenicity in Cats with Insights from Venereal Trichomonosis.Journal of veterinary internal medicineReview
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Host parasitism by Trichomonas vaginalis is complex, and the adhesion to vaginal epithelial cells (VECs) by trichomonads is preparatory to colonization of the vagina. Since we showed increased synthesis of adhesins after contact with VECs (A. F. Garcia, et al., Mol. Microbiol. 47:1207-1224, 2003) and more recently demonstrated up-regulated gene expression in VECs after parasite attachment (A. S. Kucknoor, et al., Cell. Microbiol. 7:887-897, 2005), we hypothesized that enhanced expression of adhesin and other genes would result from signaling of trichomonads following adherence. In order to identify the genes that are up-regulated, we constructed a subtraction cDNA library enriched for differentially expressed genes from the parasites that were in contact with the host cells. Thirty randomly selected cDNA clones representing the differentially regulated genes upon initial contact of parasites with host cells were sequenced. Several genes encoded functional proteins with specific functions known to be associated with colonization, such as adherence, change in morphology, and gene transcription and translation. Interestingly, genes unique to trichomonads with unknown functions were also up-regulated. Semiquantitative reverse transcription-PCR (RT-PCR) confirmed expression of select genes. An increased amount of protein was demonstrated by immunoblotting with monoclonal antibody. Finally, we showed the transcriptional regulation of some genes by iron by using RT-PCR. To our knowledge, this is the first report addressing the differential regulation of T. vaginalis genes immediately upon contact with VECs.
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