ArticleInfection and immunity2005
The Salmonella enterica serovar Typhi tsx gene, encoding a nucleoside-specific porin, is essential for prototrophic growth in the absence of nucleosides.
Article in Infection and immunity, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- New insights into the stress response mechanisms of stress-resistantEmerging microbes & infections · 2025Article
- Distinct Potentially Adaptive Accumulation of Truncation Mutations in Salmonella enterica serovar Typhi and Salmonella enterica serovar Paratyphi A.Microbiology spectrum · 2022Article
- CdsH Contributes to the Replication ofMicroorganisms · 2020Article
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- Identification of Genetic Features for Attenuation of TwoFrontiers in veterinary science · 2019Article
- Codon usage revisited: Lack of correlation between codon usage and the number of tRNA genes in enterobacteria.Biochemical and biophysical research communications · 2018Article
- Comparative proteomic analysis of outer membrane protein 43 (Journal of veterinary science · 2018Article
- Molecular detection of the index case of a subclinical Salmonella Kentucky epidemic on a dairy farm.Epidemiology and infection · 2015Article
- Draft Genome Sequence of Salmonella enterica Serovar Typhi Strain STH2370.Genome announcements · 2014Article
- Modified intracellular-associated phenotypes in a recombinant Salmonella Typhi expressing S. Typhimurium SPI-3 sequences.PloS one · 2010Article
- Comparative proteomic analysis of the Haemophilus ducreyi porin-deficient mutant 35000HP::P2AB.Journal of bacteriology · 2009Article
- Article
- The application of Tet repressor in prokaryotic gene regulation and expression.Microbial biotechnology · 2008Review
- The cotranscribed Salmonella enterica sv. Typhi tsx and impX genes encode opposing nucleoside-specific import and export proteins.Genetics · 2006Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Salmonella enterica serovar Typhi tsx gene encodes a porin that facilitates the import of nucleosides. When serovar Typhi is grown under anaerobic conditions, Tsx is among the outer membrane proteins whose expression increases dramatically. This increase in expression is due, at least in part, to increased transcription and is dependent on Fnr but not on ArcA. A mutant derivative of serovar Typhi strain STH2370 with a deletion of the tsx gene is an auxotroph that requires either adenosine or thymidine for growth on minimal medium. In contrast, an otherwise isogenic nupG nupC double mutant, defective in the inner membrane nucleoside permeases, is a prototroph. Because anaerobic growth enhances the virulence of serovar Typhi in vitro, we assessed the role that the tsx gene plays in pathogenicity and found that the serovar Typhi STH2370 Deltatsx mutant is defective in survival within human macrophage-like U937 cells. To understand why the Deltatsx mutant is an auxotroph, we selected for insertions of minitransposon T-POP in the Deltatsx genetic background that restored prototrophy. One T-POP insertion that suppressed the Deltatsx mutation in the presence of the inducer tetracycline was located upstream of the pyrD gene. The results of reverse transcription-PCR analysis showed that addition of the inducer decreased the rate of pyrD transcription. These results suggest that the Tsx porin and the balance of products of the tsx and pyrD genes play critical roles in membrane assembly and integrity and thus in the virulence of serovar Typhi.
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