ArticleRNA (New York, N.Y.)2005
Spurious splicing within the XIAP 5' UTR occurs in the Rluc/Fluc but not the betagal/CAT bicistronic reporter system.
Article in RNA (New York, N.Y.), 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it, 65 citations in OpenAlex.
- Current Practice in Bicistronic IRES Reporter Use: A Systematic Review.International journal of molecular sciences · 2021Pooled it
- Eukaryotic Initiation Factor 3F (eIF3F) Regulates the IRES-Mediated Translation of Bcl-xL via Its Interaction with Programmed Cell Death 4 (PDCD4) Protein.International journal of molecular sciences · 2026Article
- Guidelines for minimal reporting requirements, design and interpretation of experiments involving the use of eukaryotic dual gene expression reporters (MINDR).Nature structural & molecular biology · 2025Review
- A Review on Circular RNA Translation and Its Implications in Disease.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Revisiting the pathogenic mechanism of the GJB1 5' UTR c.-103C > T mutation causing CMTX1.Neurogenetics · 2021Article
- Cellular mRNA recruits the ribosome via eIF3-PABP bridge to initiate internal translation.RNA biology · 2017Article
- Translational control of PML contributes to TNFα-induced apoptosis of MCF7 breast cancer cells and decreased angiogenesis in HUVECs.Cell death and differentiation · 2016Article
- Stat1 stimulates cap-independent mRNA translation to inhibit cell proliferation and promote survival in response to antitumor drugs.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Article
- Optimal translational termination requires C4 lysyl hydroxylation of eRF1.Molecular cell · 2014Article
- The melanoma antigens MELOE-1 and MELOE-2 are translated from a bona fide polycistronic mRNA containing functional IRES sequences.PloS one · 2013Article
- Cryptic transcripts from a ubiquitous plasmid origin of replication confound tests for cis-regulatory function.Nucleic acids research · 2012Article
- Two independent mechanisms promote expression of an N-terminal truncated USP18 isoform with higher DeISGylation activity in the nucleus.The Journal of biological chemistry · 2012Article
- La enhances IRES-mediated translation of laminin B1 during malignant epithelial to mesenchymal transition.Nucleic acids research · 2012Article
- Ribosomal scanning on the 5'-untranslated region of the human immunodeficiency virus RNA genome.Nucleic acids research · 2011Article
- In vitro and in vivo comparison of viral and cellular internal ribosome entry sites for bicistronic vector expression.Gene therapy · 2011Article
- RNA-binding protein HuR mediates cytoprotection through stimulation of XIAP translation.Oncogene · 2011Article
- Distinct 5' UTRs regulate XIAP expression under normal growth conditions and during cellular stress.Nucleic acids research · 2010Article
- NF45 functions as an IRES trans-acting factor that is required for translation of cIAP1 during the unfolded protein response.Cell death and differentiation · 2010Article
- An internal ribosomal entry site mediates redox-sensitive translation of Nrf2.Nucleic acids research · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
X-chromosome-linked inhibitor of apoptosis, XIAP, has been shown to contain a strong internal ribosome entry site (IRES) within its 5' untranslated region (UTR) that promotes translation of XIAP mRNA under conditions of cellular stress. This claim came under scrutiny in a recent report demonstrating that the XIAP 5' UTR undergoes splicing when inserted between the two reporter cistrons of the dual luciferase plasmid Rluc/Fluc. In this paper, we demonstrate that the splicing within the XIAP 5' UTR specifically occurs only in the context of mRNA produced from the Rluc/Fluc but not the pbetagal/CAT bicistronic reporter plasmid.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.