ArticleRNA (New York, N.Y.)2005
Rsp5 ubiquitin ligase modulates translation accuracy in yeast Saccharomyces cerevisiae.
Article in RNA (New York, N.Y.), 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 27 citations in OpenAlex.
- Chromosome duplication causes premature aging via defects in ribosome quality control.PLoS biology · 2025Article
- Premature aging in aneuploid yeast is caused in part by aneuploidy-induced defects in Ribosome Quality Control.bioRxiv : the preprint server for biology · 2024Article
- Review
- E3 ubiquitin ligase Pub1 is implicated in endocytosis of a GPI-anchored protein Ecm33 in fission yeast.PloS one · 2014Article
- Yeast ubiquitin ligase Rsp5 contains nuclear localization and export signals.European journal of cell biology · 2011Article
- The ubiquitin ligase Rsp5 is required for ribosome stability in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2011Article
- Dual sorting of the Saccharomyces cerevisiae vacuolar protein Sna4p.Eukaryotic cell · 2009Article
- The RNA polymerase II CTD kinase Ctk1 functions in translation elongation.Genes & development · 2007Article
- Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast.Molecular systems biology · 2007Article
- Rabies virus matrix protein interplay with eIF3, new insights into rabies virus pathogenesis.Nucleic acids research · 2007Article
- Enhanced levels of Pis1p (phosphatidylinositol synthase) improve the growth of Saccharomyces cerevisiae cells deficient in Rsp5 ubiquitin ligase.The Biochemical journal · 2006Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 3 institutions in 4 countries.
Funding
Abstract
Rsp5p is an essential yeast ubiquitin protein ligase that ubiquitinates multiple proteins involved in various processes. Recent studies indicate that ubiquitination also affects translation. Here, we show that the strain with the rsp5-13 mutation exhibits altered sensitivity to antibiotics and a slower rate of translation. Using a sensitive dual-gene reporter system, we demonstrate that stop codon readthrough efficiency is decreased in the rsp5-13 mutant, while both +1 and -1 frameshifting were unaffected. The effect of the rsp5-13 mutation on readthrough could be reversed by increased expression of ubiquitin and partially suppressed by overproduction of the elongation factor eEF1A. As assessed by fluorescence in situ hybridization, the rsp5-13 mutant cells accumulate tRNA nuclear pools, perhaps depleting tRNA from the cytoplasm. Nuclear accumulation of tRNA is observed only when rsp5-13 cells are grown in media with high amino acid content. This defect, also reversed by overproduction of the elongation factor eEF1A, may be the primary reason for altered translational decoding accuracy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.