ArticleMolecular biology of the cell2005
The chromokinesin Kid is required for maintenance of proper metaphase spindle size.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Novel KIF22 Variants Disrupt Mitosis in Human Chondrocytes and Expand SEMDJL2 Mechanisms.bioRxiv : the preprint server for biology · 2026Article
- New insights into the molecular basis of gametogenesis in the hybridogenetic water frog Pelophylax esculentus.Scientific reports · 2026Article
- The kinetochore corona orchestrates chromosome congression through transient microtubule interactions.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Article
- Understanding copy number variations through their genes: a molecular view on 16p11.2 deletion and duplication syndromes.Frontiers in pharmacology · 2024Review
- Pathogenic mutations in the chromokinesin KIF22 disrupt anaphase chromosome segregation.eLife · 2022Article
- Kinesin Motors in the Filamentous Basidiomycetes in Light of theJournal of fungi (Basel, Switzerland) · 2022Review
- Expression of Genes in the 16p11.2 Locus during Development of the Human Fetal Cerebral Cortex.Cerebral cortex (New York, N.Y. : 1991) · 2021Article
- The developmental biology of kinesins.Developmental biology · 2021Review
- The functions of kinesin and kinesin-related proteins in eukaryotes.Cell adhesion & migration · 2020Review
- Induction of a Spindle-Assembly-Competent M Phase in Xenopus Egg Extracts.Current biology : CB · 2019Article
- Delayed Chromosome Alignment to the Spindle Equator Increases the Rate of Chromosome Missegregation in Cancer Cell Lines.Biomolecules · 2018Article
- NOD is a plus end-directed motor that binds EB1 via a new microtubule tip localization sequence.The Journal of cell biology · 2018Article
- KIF22 coordinates CAR and EGFR dynamics to promote cancer cell proliferation.Science signaling · 2018Article
- Eml1 loss impairs apical progenitor spindle length and soma shape in the developing cerebral cortex.Scientific reports · 2017Article
- Mechanisms of Chromosome Congression during Mitosis.Biology · 2017Review
- Length-dependent anisotropic scaling of spindle shape.Biology open · 2014Article
- Chromosome position at the spindle equator is regulated by chromokinesin and a bipolar microtubule array.Scientific reports · 2013Article
- Elevated polar ejection forces stabilize kinetochore-microtubule attachments.The Journal of cell biology · 2013Article
- Human chromokinesins promote chromosome congression and spindle microtubule dynamics during mitosis.The Journal of cell biology · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human chromokinesin Kid/kinesin-10, a plus end-directed microtubule (MT)-based motor with both microtubule- and DNA-binding domains, is required for proper chromosome alignment at the metaphase plate. Here, we performed RNA interference experiments to deplete endogenous Kid from HeLa cells and confirmed defects in metaphase chromosome arm alignment in Kid-depleted cells. In addition, we noted a shortening of the spindle length, resulting in a pole-to-pole distance only 80% of wild type. The spindle microtubule-bundles with which Kid normally colocalize became less robust. Rescue of the two Kid deficiency phenotypes-imprecise chromosome alignment at metaphase and shortened spindles- exhibited distinct requirements. Mutants lacking either the DNA-binding domain or the MT motor ATPase failed to rescue the former defect, whereas rescue of the shortened spindle phenotype required neither activity. Kid also exhibits microtubule bundling activity in vitro, and rescue of the shortened spindle phenotype and the bundling activity displayed similar domain requirements, except that rescue required a coiled-coil domain not needed for bundling. These results suggest that distinct from its role in chromosome movement, Kid contributes to spindle morphogenesis by mediating spindle microtubules stabilization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.