ArticleMolecular biology of the cell2005
Ras mutation impairs epithelial barrier function to a wide range of nonelectrolytes.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- Co-trimoxazole-induced reproductive toxicity and placental-barrier disruption: impact on cell-cell junctions and ERK signaling pathway.Frontiers in cell and developmental biology · 2026Article
- Chitosan Oligosaccharide Promotes Junction Barrier through Modulation of PI3K/AKT and ERK Signaling Intricate Interplay in T84 Cells.Polymers · 2023Article
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- HDAC-4 regulates claudin-2 expression in EGFR-ERK1/2 dependent manner to regulate colonic epithelial cell differentiation.Oncotarget · 2017Article
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- Methionine restriction fundamentally supports health by tightening epithelial barriers.Annals of the New York Academy of Sciences · 2016Review
- Cellular polarity in aging: role of redox regulation and nutrition.Genes & nutrition · 2014Article
- Tight junctions in lung cancer and lung metastasis: a review.International journal of clinical and experimental pathology · 2012Review
- Claudins in lung diseases.Respiratory research · 2011Review
- Contrasting effects of ERK on tight junction integrity in differentiated and under-differentiated Caco-2 cell monolayers.The Biochemical journal · 2011Article
- Dietary methionine restriction improves colon tight junction barrier function and alters claudin expression pattern.American journal of physiology. Cell physiology · 2010Article
- Ouabain modulates epithelial cell tight junction.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Transmucosal gastric leak induced by proton pump inhibitors.Digestive diseases and sciences · 2009Article
- The transforming Rho family GTPase Wrch-1 disrupts epithelial cell tight junctions and epithelial morphogenesis.Molecular and cellular biology · 2009Article
- Transepithelial leak in Barrett's esophagus.Digestive diseases and sciences · 2006Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although ras mutations have been shown to affect epithelial architecture and polarity, their role in altering tight junctions remains unclear. Transfection of a valine-12 mutated ras construct into LLC-PK1 renal epithelia produces leakiness of tight junctions to certain types of solutes. Transepithelial permeability of D-mannitol increases sixfold but transepithelial electrical resistance increases >40%. This indicates decreased paracellular permeability to NaCl but increased permeability to nonelectrolytes. Permeability increases to D-mannitol (Mr 182), polyethylene glycol (Mr 4000), and 10,000-Mr methylated dextran but not to 2,000,000-Mr methylated dextran. This implies a "ceiling" on the size of solutes that can cross a ras-mutated epithelial barrier and therefore that the increased permeability is not due to loss of cells or junctions. Although the abundance of claudin-2 declined to undetectable levels in the ras-overexpressing cells compared with vector controls, levels of occludin and claudins 1, 4, and 7 increased. The abundance of claudins-3 and -5 remained unchanged. An increase in extracellular signal-regulated kinase-2 phosphorylation suggests that the downstream effects on the tight junction may be due to changes in the mitogen-activated protein kinase signaling pathway. These selective changes in permeability may influence tumorigenesis by the types of solutes now able to cross the epithelial barrier.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.