ArticleProceedings of the National Academy of Sciences of the United States of America2005
Systematic yeast synthetic lethal and synthetic dosage lethal screens identify genes required for chromosome segregation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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76 citing papers in PubMed, 136 citations in OpenAlex.
- Genetic-interaction screens uncover novel biological roles and regulators of transcription factors in fission yeast.G3 (Bethesda, Md.) · 2022Article
- VID22 counteracts G-quadruplex-induced genome instability.Nucleic acids research · 2021Article
- CHIP and BAP1 Act in Concert to Regulate INO80 Ubiquitination and Stability for DNA Replication.Molecules and cells · 2021Article
- Mapping Synthetic Dosage Lethal Genetic Interactions in Saccharomyces cerevisiae.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The role of gene dosage in budding yeast centrosome scaling and spontaneous diploidization.PLoS genetics · 2020Article
- A genetic interaction map centered on cohesin reveals auxiliary factors involved in sister chromatid cohesion inJournal of cell science · 2020Article
- CATS: Cas9-assisted tag switching. A high-throughput method for exchanging genomic peptide tags in yeast.BMC genomics · 2020Article
- Functional Characterization of a Novel Oxidative Stress Protection Protein in the Pathogenic YeastFrontiers in genetics · 2020Article
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- Flux balance analysis with or without molecular crowding fails to predict two thirds of experimentally observed epistasis in yeast.Scientific reports · 2019Article
- Yeast genetic interaction screens in the age of CRISPR/Cas.Current genetics · 2019Review
- Stu2 uses a 15-nm parallel coiled coil for kinetochore localization and concomitant regulation of the mitotic spindle.Molecular biology of the cell · 2018Article
- Mapping a diversity of genetic interactions in yeast.Current opinion in systems biology · 2017Article
- Pib2 and the EGO complex are both required for activation of TORC1.Journal of cell science · 2017Article
- Genome maintenance functions of the INO80 chromatin remodeller.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Mapping the Synthetic Dosage Lethality Network ofG3 (Bethesda, Md.) · 2017Article
- Haploid yeast cells undergo a reversible phenotypic switch associated with chromosome II copy number.BMC genetics · 2016Article
- The interplay between chromosome stability and cell cycle control explored through gene-gene interaction and computational simulation.Nucleic acids research · 2016Article
- Overexpression screens identify conserved dosage chromosome instability genes in yeast and human cancer.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
16 more citing papers are in PubMed but not listed here.
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16 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accurate chromosome segregation requires the execution and coordination of many processes during mitosis, including DNA replication, sister chromatid cohesion, and attachment of chromosomes to spindle microtubules via the kinetochore complex. Additional pathways are likely involved because faithful chromosome segregation also requires proteins that are not physically associated with the chromosome. Using kinetochore mutants as a starting point, we have identified genes with roles in chromosome stability by performing genome-wide screens employing synthetic genetic array methodology. Two genetic approaches (a series of synthetic lethal and synthetic dosage lethal screens) isolated 211 nonessential deletion mutants that were unable to tolerate defects in kinetochore function. Although synthetic lethality and synthetic dosage lethality are thought to be based upon similar genetic principles, we found that the majority of interactions associated with these two screens were nonoverlapping. To functionally characterize genes isolated in our screens, a secondary screen was performed to assess defects in chromosome segregation. Genes identified in the secondary screen were enriched for genes with known roles in chromosome segregation. We also uncovered genes with diverse functions, such as RCS1, which encodes an iron transcription factor. RCS1 was one of a small group of genes identified in all three screens, and we used genetic and cell biological assays to confirm that it is required for chromosome stability. Our study shows that systematic genetic screens are a powerful means to discover roles for uncharacterized genes and genes with alternative functions in chromosome maintenance that may not be discovered by using proteomics approaches.
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