ArticleMammalian genome : official journal of the International Mammalian Genome Society1992
Multilocus markers for mouse genome analysis: PCR amplification based on single primers of arbitrary nucleotide sequence.
Article in Mammalian genome : official journal of the International Mammalian Genome Society, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Genotypes for 66 microsatellite markers in the AXB and BXA recombinant inbred mouse strains.Mammalian genome : official journal of the International Mammalian Genome Society · 1998Article
- An additional 136 SSLP markers typed for the AXB and BXA recombinant inbred mouse strains.Mammalian genome : official journal of the International Mammalian Genome Society · 1997Article
- Identification and genetic mapping of random amplified polymorphic DNA (RAPD) markers to the sheep genome.Mammalian genome : official journal of the International Mammalian Genome Society · 1996Article
- Mapping new murine polymorphisms detected by random amplified polymorphic DNA (RAPD) PCR.Mammalian genome : official journal of the International Mammalian Genome Society · 1996Article
- Fine genetic mapping of the region surrounding the high growth (hg) locus in mouse chromosome 10: targeting random amplified polymorphic DNA (RAPD) markers.Mammalian genome : official journal of the International Mammalian Genome Society · 1996Article
- Mapping the mouse genome: current status and future prospects.Proceedings of the National Academy of Sciences of the United States of America · 1995Review
- New murine polymorphisms detected by random amplified polymorphic DNA (RAPD) PCR and mapped by use of recombinant inbred strains.Mammalian genome : official journal of the International Mammalian Genome Society · 1994Article
- MAAP: a versatile and universal tool for genome analysis.Plant molecular biology · 1994Article
- Maps from two interspecific backcross DNA panels available as a community genetic mapping resource.Mammalian genome : official journal of the International Mammalian Genome Society · 1994Article
- Evaluating two methods for fingerprinting genomes of Actinobacillus actinomycetemcomitans.Oral microbiology and immunology · 1993Article
- A genetic linkage map of Saccharum spontaneum L. 'SES 208'.Genetics · 1993Article
- Murine c-mpl: a member of the hematopoietic growth factor receptor superfamily that transduces a proliferative signal.The EMBO journal · 1993Article
- Genomic mapping of intracisternal A-particle proviral elements.Mammalian genome : official journal of the International Mammalian Genome Society · 1993Article
- Genome mapping by arbitrary amplification of yeast artificial chromosomes.Mammalian genome : official journal of the International Mammalian Genome Society · 1993Article
- The identification of Y chromosome-linked markers with random sequence oligonucleotide primers.Mammalian genome : official journal of the International Mammalian Genome Society · 1993Article
- Arbitrarily primed PCR fingerprinting of RNA.Nucleic acids research · 1992Article
- Mouse chromosome 14.Mammalian genome : official journal of the International Mammalian Genome Society · 1992Review
- Mouse chromosome 3.Mammalian genome : official journal of the International Mammalian Genome Society · 1992Review
- Mouse chromosome 19.Mammalian genome : official journal of the International Mammalian Genome Society · 1992Review
- Mouse chromosome 12.Mammalian genome : official journal of the International Mammalian Genome Society · 1992Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Polymerase chain reaction (PCR) based on single primers of arbitrary nucleotide sequence provides a powerful marker system for genome analysis because each primer amplifies multiple products, and cloning, sequencing, and hybridization are not required. We have evaluated this typing system for the mouse by identifying optimal PCR conditions; characterizing effects of GC content, primer length, and multiplexed primers; demonstrating considerable variation among a panel of inbred strains; and establishing linkage for several products. Mg2+, primer, template, and annealing conditions were identified that optimized the number and resolution of amplified products. Primers with 40% GC content failed to amplify products readily, primers with 50% GC content resulted in reasonable amplification, and primers with 60% GC content gave the largest number of well-resolved products. Longer primers did not necessarily amplify more products than shorter primers of the same proportional GC content. Multiplexed primers yielded more products than either primer alone and usually revealed novel variants. A strain survey showed that most strains could be readily distinguished with a modest number of primers. Finally, linkage for seven products was established on five chromosomes. These characteristics establish single primer PCR as a powerful method for mouse genome analysis.
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