ArticleOncogene2006
Cytokinesis regulator ECT2 changes its conformation through phosphorylation at Thr-341 in G2/M phase.
Article in Oncogene, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 78 citations in OpenAlex.
- Differential roles of cyclin-CDK1 complexes in cell migration and invasion.Journal of cell science · 2025Article
- Inference on autoregulation in gene expression with variance-to-mean ratio.Journal of mathematical biology · 2023Article
- Interphase microtubule disassembly is a signaling cue that drives cell rounding at mitotic entry.The Journal of cell biology · 2022Article
- Diversity is the spice of life: An overview of how cytokinesis regulation varies with cell type.Frontiers in cell and developmental biology · 2022Review
- Complementary functions for the Ran gradient during division.Small GTPases · 2021Review
- ECT2 promotes lung adenocarcinoma progression through extracellular matrix dynamics and focal adhesion signaling.Cancer science · 2021Article
- Structure and regulation of human epithelial cell transforming 2 protein.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis.Theranostics · 2020Article
- Effect of Ect2 Expression on the Growth of Triple-Negative Breast Cancer Cells with Paclitaxel Intervention.OncoTargets and therapy · 2020Article
- Molecular Mechanism of Cytokinesis.Annual review of biochemistry · 2019Review
- Polymerase-δ-interacting protein 2 activates the RhoGEF epithelial cell transforming sequence 2 in vascular smooth muscle cells.American journal of physiology. Cell physiology · 2019Article
- Cytoplasmic expression of epithelial cell transforming sequence 2 in lung adenocarcinoma and its implications for malignant progression.Laboratory investigation; a journal of technical methods and pathology · 2019Article
- Modulation of alternative splicing induced by paclitaxel in human lung cancer.Cell death & disease · 2018Article
- Article
- Role of Epithelial Cell Transforming Sequence 2 (ECT2) in Predicting Prognosis of Osteosarcoma.Medical science monitor : international medical journal of experimental and clinical research · 2017Article
- P53 and Protein Phosphorylation Regulate the Oncogenic Role of Epithelial Cell Transforming 2 (ECT2).Medical science monitor : international medical journal of experimental and clinical research · 2017Article
- Early Assessment of Colorectal Cancer by Quantifying Circulating Tumor Cells in Peripheral Blood: ECT2 in Diagnosis of Colorectal Cancer.International journal of molecular sciences · 2017Article
- Ect2-Dependent rRNA Synthesis Is Required for KRAS-TRP53-Driven Lung Adenocarcinoma.Cancer cell · 2017Article
- Ect2/Pbl acts via Rho and polarity proteins to direct the assembly of an isotropic actomyosin cortex upon mitotic entry.Developmental cell · 2015Article
- Controlling the switches: Rho GTPase regulation during animal cell mitosis.Cellular signalling · 2014Review
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The Rho activator ECT2 functions as a key regulator in cytokinesis. ECT2 is phosphorylated during G2/M phase, but the physiological significance of this event is not well known. In this study, we show that phosphorylation of ECT2 at threonine-341 (T341) affects the autoregulatory mechanism of ECT2. In G2/M phase, ECT2 was phosphorylated at T341 most likely by Cyclin B/Cyclin-dependent kinase 1 (Cdk1), and then dephosphorylated before cytokinesis. Depletion of ECT2 by RNA interference (RNAi) efficiently induced multinucleate cells. Expression of the phospho-deficient mutant of ECT2 at T341 suppressed the multinucleation induced by RNAi to ECT2, indicating that ECT2 is biologically active even when it is not phosphorylated at T341. However, the phospho-mimic mutation at T341 weakly stimulates the catalytic activity of ECT2 as detected by serum response element reporter gene assays. As T341 is located at the hinge region of the N-terminal regulatory domain and C-terminal catalytic domain, phosphorylation of T341 may help accessing downstream signaling molecules to further activate ECT2. We found that the phospho-mimic mutation T341D increases binding with itself or the N-terminal half of ECT2. These results suggest a conformational change of ECT2 upon phosphorylation at T341. Therefore, ECT2 activity might be regulated by the phosphorylation status of T341. We propose that T341 phosphorylation by Cyclin B/Cdk1 could be a trigger for further activation of ECT2.
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