ArticleNucleic acids research2005
Binding of serum response factor to cystic fibrosis transmembrane conductance regulator CArG-like elements, as a new potential CFTR transcriptional regulation pathway.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Long non-coding RNAs in the CFTR locus: New transcriptional regulators of CFTR gene expression.Molecular therapy. Nucleic acids · 2026Article
- Glia maturation factor beta deficiency protects against diabetic osteoporosis by suppressing osteoclast hyperactivity.Experimental & molecular medicine · 2023Article
- CFTR Lifecycle Map-A Systems Medicine Model of CFTR Maturation to Predict Possible Active Compound Combinations.International journal of molecular sciences · 2021Article
- Article
- A balance between activating and repressive histone modifications regulates cystic fibrosis transmembrane conductance regulator (CFTR) expression in vivo.Epigenetics · 2014Article
- Phosphorylated C/EBPβ influences a complex network involving YY1 and USF2 in lung epithelial cells.PloS one · 2013Article
- Nucleosome occupancy reveals regulatory elements of the CFTR promoter.Nucleic acids research · 2012Article
- p.Ser1235Arg should no longer be considered as a cystic fibrosis mutation: results from a large collaborative study.European journal of human genetics : EJHG · 2011Article
- Multiple mechanisms influence regulation of the cystic fibrosis transmembrane conductance regulator gene promoter.American journal of respiratory cell and molecular biology · 2010Article
- NF-E2-related factor 2, a key inducer of antioxidant defenses, negatively regulates the CFTR transcription.Cellular and molecular life sciences : CMLS · 2010Article
- The smooth muscle cell-restricted KCNMB1 ion channel subunit is a direct transcriptional target of serum response factor and myocardin.The Journal of biological chemistry · 2009Article
- Homozygosity for a null allele of COL3A1 results in recessive Ehlers-Danlos syndrome.European journal of human genetics : EJHG · 2009Article
- A complex intronic enhancer regulates expression of the CFTR gene by direct interaction with the promoter.Journal of cellular and molecular medicine · 2009Article
- Serum response factor binding sites differ in three human cell types.Genome research · 2007Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CFTR expression is tightly controlled by a complex network of ubiquitous and tissue-specific cis-elements and trans-factors. To better understand mechanisms that regulate transcription of CFTR, we examined transcription factors that specifically bind a CFTR CArG-like motif we have previously shown to modulate CFTR expression. Gel mobility shift assays and chromatin immunoprecipitation analyses demonstrated the CFTR CArG-like motif binds serum response factor both in vitro and in vivo. Transient co-transfections with various SRF expression vector, including dominant-negative forms and small interfering RNA, demonstrated that SRF significantly increases CFTR transcriptional activity in bronchial epithelial cells. Mutagenesis studies suggested that in addition to SRF other co-factors, such as Yin Yang 1 (YY1) previously shown to bind the CFTR promoter, are potentially involved in the CFTR regulation. Here, we show that functional interplay between SRF and YY1 might provide interesting perspectives to further characterize the underlying molecular mechanism of the basal CFTR transcriptional activity. Furthermore, the identification of multiple CArG binding sites in highly conserved CFTR untranslated regions, which form specific SRF complexes, provides direct evidence for a considerable role of SRF in the CFTR transcriptional regulation into specialized epithelial lung cells.
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