ArticleBiophysical journal2005
Fluorescence lifetime heterogeneity resolution in the frequency domain by lifetime moments analysis.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications.Journal of biomedical optics · 2020Review
- High-throughput, multi-parametric, and correlative fluorescence lifetime imaging.Methods and applications in fluorescence · 2020Review
- Chromatin nanoscale compaction in live cells visualized by acceptor-to-donor ratio corrected Förster resonance energy transfer between DNA dyes.Journal of biophotonics · 2019Article
- Discovery of High-Affinity Noncovalent Allosteric KRAS Inhibitors That Disrupt Effector Binding.ACS omega · 2019Article
- Rapid fluorescence lifetime estimation with modified phasor approach and Laguerre deconvolution: a comparative study.Methods and applications in fluorescence · 2017Article
- Intensity correlation-based calibration of FRET.Biophysical journal · 2013Article
- Subcellular localization-dependent changes in EGFP fluorescence lifetime measured by time-resolved flow cytometry.Biomedical optics express · 2013Article
- Rotaviruses associate with cellular lipid droplet components to replicate in viroplasms, and compounds disrupting or blocking lipid droplets inhibit viroplasm formation and viral replication.Journal of virology · 2010Article
- BAG1 restores formation of functional DJ-1 L166P dimers and DJ-1 chaperone activity.The Journal of cell biology · 2010Article
- The potential of optical proteomic technologies to individualize prognosis and guide rational treatment for cancer patients.Targeted oncology · 2009Review
- Neuroprotective secreted amyloid precursor protein acts by disrupting amyloid precursor protein dimers.The Journal of biological chemistry · 2009Article
- Quantitative FRET analysis by fast acquisition time domain FLIM at high spatial resolution in living cells.Biophysical journal · 2008Article
- Fibroblast growth factor-regulated palmitoylation of the neural cell adhesion molecule determines neuronal morphogenesis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- K-ras nanoclustering is subverted by overexpression of the scaffold protein galectin-3.Cancer research · 2008Article
- Electrostatic interactions positively regulate K-Ras nanocluster formation and function.Molecular and cellular biology · 2008Article
- Quantitative lifetime unmixing of multiexponentially decaying fluorophores using single-frequency fluorescence lifetime imaging microscopy.Biophysical journal · 2008Article
- Galectin-1 is a novel structural component and a major regulator of h-ras nanoclusters.Molecular biology of the cell · 2008Article
- Flotillin-dependent clustering of the amyloid precursor protein regulates its endocytosis and amyloidogenic processing in neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Quantitative analysis of fluorescence lifetime imaging made easy.HFSP journal · 2008Article
- Molecular fluorescence, phosphorescence, and chemiluminescence spectrometry.Analytical chemistry · 2006Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence lifetime imaging microscopy presents a powerful tool in biology and biophysics because it allows the investigation of the local environment of a fluorochrome in living cells in a quantitative manner. Furthermore, imaging Förster-type resonance energy transfer (FRET) by fluorescence lifetime imaging microscopy enables protein-protein interactions and intermolecular distances to be mapped under physiological conditions. Quantitative and precise data analysis methods are required to access the richness of information that is contained in FRET data on biological samples. Lifetime detection in the frequency-domain yields two lifetime estimations. The lifetime moments analysis (LiMA) provides a quantitative measure of the lifetime distribution broadness by exploiting the analytical relationship between the phase- and demodulation-lifetime estimations and relating them to the weighted average and variance of the lifetime distribution. The LiMA theoretical framework is validated by comparison with global analysis and by applying it to a constrained two-component FRET system using simulations and experiments. Furthermore, a novel LIMA-based error analysis and a more intuitive formalism for global analysis are presented. Finally, a new method to resolve a FRET system is proposed and experimentally applied to the investigation of protein-protein interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.