ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2005
Intron-flanking EST-PCR markers: from genetic marker development to gene structure analysis in Rhododendron.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 67 citations in OpenAlex.
- Review
- Development of Intron Polymorphism Markers and Their Association With Fatty Acid Component Variation in Oil Palm.Frontiers in plant science · 2022Article
- Demographic history and adaptive synonymous and nonsynonymous variants of nuclear genes in Rhododendron oldhamii (Ericaceae).Scientific reports · 2020Article
- Identification and Characterization of Five Cold Stress-Related Rhododendron Dehydrin Genes: Spotlight on a FSK-Type Dehydrin With Multiple F-Segments.Frontiers in bioengineering and biotechnology · 2019Article
- Genome-wide analysis of SSR and ILP markers in trees: diversity profiling, alternate distribution, and applications in duplication.Scientific reports · 2017Article
- Proteome dynamics of cold-acclimating Rhododendron species contrasting in their freezing tolerance and thermonasty behavior.PloS one · 2017Article
- Population demographic history of a temperate shrub,Ecology and evolution · 2016Article
- Persistent history of the bird-dispersed arctic-alpine plant Vaccinium vitis-idaea L. (Ericaceae) in Japan.Journal of plant research · 2015Article
- Advances in plant gene-targeted and functional markers: a review.Plant methods · 2013Article
- Exploiting EST databases for the development and characterisation of 3425 gene-tagged CISP markers in biofuel crop sugarcane and their transferability in cereals and orphan tropical grasses.BMC research notes · 2013Article
- Fine mapping of wheat stripe rust resistance gene Yr26 based on collinearity of wheat with Brachypodium distachyon and rice.PloS one · 2013Article
- Development and characterization of intron-flanking EST-PCR markers in rubber tree (Hevea brasiliensis Muell. Arg.).Molecular biotechnology · 2012Article
- EST-derived genic molecular markers: development and utilization for generating an advanced transcript map of chickpea.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Collinearity-based marker mining for the fine mapping of Pm6, a powdery mildew resistance gene in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011Article
- Discovery of intron polymorphisms in cultivated tomato using both tomato and Arabidopsis genomic information.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010Article
- Development of EST-PCR markers for Thinopyrum intermedium chromosome 2Ai#2 and their application in characterization of novel wheat-grass recombinants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2010Article
- Multipoint-likelihood maximization mapping on 4 segregating populations to achieve an integrated framework map for QTL analysis in pot azalea (Rhododendron simsii hybrids).BMC molecular biology · 2010Article
- ConservedPrimers 2.0: a high-throughput pipeline for comparative genome referenced intron-flanking PCR primer design and its application in wheat SNP discovery.BMC bioinformatics · 2009Article
- Development of intron-flanking EST markers for the Lolium/Festuca complex using rice genomic information.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- Localization of anchor loci representing five hundred annotated rice genes to wheat chromosomes using PLUG markers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With a long-term goal of constructing a linkage map of Rhododendron enriched with gene-specific markers, we utilized Rhododendron catawbiense ESTs for the development of high-efficiency (in terms of generating polymorphism frequency) PCR-based markers. Using the gene-sequence alignment between Rhododendron ESTs and the genomic sequences of Arabidopsis homologs, we developed 'intron-flanking' EST-PCR-based primers that would anneal in conserved exon regions and amplify across the more highly diverged introns. These primers resulted in increased efficiency (61% vs. 13%; 4.7-fold) of polymorphism-detection compared with conventional EST-PCR methods, supporting the assumption that intron regions are more diverged than exons. Significantly, this study demonstrates that Arabidopsis genome database can be useful in developing gene-specific PCR-based markers for other non-model plant species for which the EST data are available but genomic sequences are not. The comparative analysis of intron sizes between Rhododendron and Arabidopsis (made possible in this study by aligning of Rhododendron ESTs with Arabidopsis genomic sequences and the sequencing of Rhododendron genomic PCR products) provides the first insight into the gene structure of Rhododendron.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.