ArticleNucleic acids research2005
Deacylated tRNA is released from the E site upon A site occupation but before GTP is hydrolyzed by EF-Tu.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
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- Development of a Novel Amplifiable System to Quantify Hydrogen Peroxide in Living Cells.Journal of the American Chemical Society · 2024Article
- Chloramphenicol Derivatization in Its Primary Hydroxyl Group with Basic Amino Acids Leads to New Pharmacophores with High Antimicrobial Activity.Antibiotics (Basel, Switzerland) · 2023Article
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- tRNA Fluctuations Observed on Stalled Ribosomes Are Suppressed during Ongoing Protein Synthesis.Biophysical journal · 2017Article
- Article
- Dynamic relationships between ribosomal conformational and RNA positional changes during ribosomal translocation.Heliyon · 2016Article
- Model of the pathway of -1 frameshifting: Kinetics.Biochemistry and biophysics reports · 2016Article
- EF-G and EF4: translocation and back-translocation on the bacterial ribosome.Nature reviews. Microbiology · 2014Review
- Real-time assay for testing components of protein synthesis.Nucleic acids research · 2012Article
- A flexible loop in yeast ribosomal protein L11 coordinates P-site tRNA binding.Nucleic acids research · 2010Article
- The role of L1 stalk-tRNA interaction in the ribosome elongation cycle.Journal of molecular biology · 2010Article
- RPS25 is essential for translation initiation by the Dicistroviridae and hepatitis C viral IRESs.Genes & development · 2009Article
- Navigating the ribosome's metastable energy landscape.Trends in biochemical sciences · 2009Review
- Frameshifting RNA pseudoknots: structure and mechanism.Virus research · 2009Review
- Shine-Dalgarno interaction prevents incorporation of noncognate amino acids at the codon following the AUG.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Features of 80S mammalian ribosome and its subunits.Nucleic acids research · 2008Article
- A new kinetic model reveals the synergistic effect of E-, P- and A-sites on +1 ribosomal frameshifting.Nucleic acids research · 2008Article
- Genetic analysis of the E site during RF2 programmed frameshifting.RNA (New York, N.Y.) · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The presence or absence of deacylated tRNA at the E site sharply influences the activation energy required for binding of a ternary complex to the ribosomal A site indicating the different conformations that the E-tRNA imparts on the ribosome. Here we address two questions: (i) whether or not peptidyltransferase--the essential catalytic activity of the large ribosomal subunit--also depends on the occupancy state of the E site and (ii) at what stage the E-tRNA is released during an elongation cycle. Kinetics of the puromycin reaction on various functional states of the ribosome indicate that the A-site substrate of the peptidyltransferase center, puromycin, requires the same activation energy for peptide-bond formation under all conditions tested. We further demonstrate that deacylated tRNA is released from the E site by binding a ternary complex aminoacyl-tRNA*EF-Tu*GDPNP to the A site. This observation indicates that the E-tRNA is released after the decoding step but before both GTP hydrolysis by EF-Tu and accommodation of the A-tRNA. Collectively these results reveal that the reciprocal linkage between the E and A sites affects the decoding center on the 30S subunit, but does not influence the rate of peptide-bond formation at the active center of the 50S subunit.
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