ArticleNucleic acids research2005
Improved statistical analysis of budding yeast TAG microarrays revealed by defined spike-in pools.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- A multiplexed, three-dimensional pooling and next-generation sequencing strategy for creating barcoded mutant arrays: construction of a Schizosaccharomyces pombe transposon insertion library.Nucleic acids research · 2022Article
- Identification of human intracellular targets of the medicinal Herb St. John's Wort by chemical-genetic profiling in yeast.Journal of agricultural and food chemistry · 2008Article
- Systematic validation and atomic force microscopy of non-covalent short oligonucleotide barcode microarrays.PloS one · 2008Article
- TagSmart: analysis and visualization for yeast mutant fitness data measured by tag microarrays.BMC bioinformatics · 2007Article
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Authors and funding
7 authors.
Funding
Abstract
Saccharomyces cerevisiae knockout collection TAG microarrays are an emergent platform for rapid, genome-wide functional characterization of yeast genes. TAG arrays report abundance of unique oligonucleotide 'TAG' sequences incorporated into each deletion mutation of the yeast knockout collection, allowing measurement of relative strain representation across experimental conditions for all knockout mutants simultaneously. One application of TAG arrays is to perform genome-wide synthetic lethality screens, known as synthetic lethality analyzed by microarray (SLAM). We designed a fully defined spike-in pool to resemble typical SLAM experiments and performed TAG microarray hybridizations. We describe a method for analyzing two-color array data to efficiently measure the differential knockout strain representation across two experimental conditions, and use the spike-in pool to show that the sensitivity and specificity of this method exceed typical current approaches.
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