ArticleMolecular and cellular biology2005
The mating type switch-activating protein Sap1 Is required for replication fork arrest at the rRNA genes of fission yeast.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 53 citations in OpenAlex.
- The Fission Yeast Mating-Type Switching Motto: "One-for-Two" and "Two-for-One".Microbiology and molecular biology reviews : MMBR · 2023Review
- Role and Regulation of Pif1 Family Helicases at the Replication Fork.International journal of molecular sciences · 2022Review
- Structure of the replication regulator Sap1 reveals functionally important interfaces.Scientific reports · 2018Article
- Molecular signature of the imprintosome complex at the mating-type locus in fission yeast.Microbial cell (Graz, Austria) · 2018Article
- The abundance of Fob1 modulates the efficiency of rRFBs to stall replication forks.Nucleic acids research · 2017Article
- The functions of the multi-tasking Pfh1Current genetics · 2017Review
- Shelterin components mediate genome reorganization in response to replication stress.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
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- Phosphorylation of CMG helicase and Tof1 is required for programmed fork arrest.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Single-Nucleotide-Specific Targeting of the Tf1 Retrotransposon Promoted by the DNA-Binding Protein Sap1 of Schizosaccharomyces pombe.Genetics · 2015Article
- An Ancient Yeast for Young Geneticists: A Primer on the Schizosaccharomyces pombe Model System.Genetics · 2015Review
- Arrested replication forks guide retrotransposon integration.Science (New York, N.Y.) · 2015Article
- High Confidence Fission Yeast SUMO Conjugates Identified by Tandem Denaturing Affinity Purification.Scientific reports · 2015Article
- The Long Terminal Repeat Retrotransposons Tf1 and Tf2 of Schizosaccharomyces pombe.Microbiology spectrum · 2015Review
- The DNA-Binding Domain of S. pombe Mrc1 (Claspin) Acts to Enhance Stalling at Replication Barriers.PloS one · 2015Article
- Mechanism and physiological significance of programmed replication termination.Seminars in cell & developmental biology · 2014Review
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- Lsd1 and lsd2 control programmed replication fork pauses and imprinting in fission yeast.Cell reports · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Schizosaccharomyces pombe rRNA genes contain three replication fork barriers (RFB1-3) located in the nontranscribed spacer. RFB2 and RFB3 require binding of the transcription terminator factor Reb1p to two identical recognition sequences that colocalize with these barriers. RFB1, which is the strongest of the three barriers, functions in a Reb1p-independent manner, and cognate DNA-binding proteins for this barrier have not been identified yet. Here we functionally define RFB1 within a 78-bp sequence located near the 3' end of the rRNA coding region. A protein that specifically binds to this sequence was purified by affinity chromatography and identified as Sap1p by mass spectrometry. Specific binding to RFB1 was confirmed by using Sap1p expressed in Escherichia coli. Sap1p is essential for viability and is required for efficient mating-type switching. Mutations in RFB1 that precluded formation of the Sap1p-RFB1 complex systematically abolished replication barrier function, indicating that Sap1p is required for replication fork blockage at RFB1.
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