ArticleMolecular and cellular biology2005
Regulation of cell polarity by interactions of Msb3 and Msb4 with Cdc42 and polarisome components.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 70 citations in OpenAlex.
- Mitochondria-lysosome coupling contributes to lysosome acidification and aging.Molecular cell · 2026Article
- A short linear motif, conserved from yeast to human, binds to members of the Spa2 family of cortical scaffold proteins.Journal of cell science · 2026Article
- Oligomerization and exocyst coupling underlie Spa2-mediated focusing of polarized growth in fission yeast.Journal of cell science · 2025Article
- Transient septin sumoylation steers a Fir1-Skt5 protein complex between the split septin ring.The Journal of cell biology · 2024Article
- A positive feedback loop involving the Spa2 SHD domain contributes to focal polarization.PloS one · 2022Article
- Essential role of the endocytic site-associated protein Ecm25 in stress-induced cell elongation.Cell reports · 2021Article
- Genomic and Targeted Approaches Unveil the Cell Membrane as a Major Target of the Antifungal Cytotoxin Amantelide A.Chembiochem : a European journal of chemical biology · 2021Article
- Type V myosin focuses the polarisome and shapes the tip of yeast cells.The Journal of cell biology · 2021Article
- Spitzenkörper assembly mechanisms reveal conserved features of fungal and metazoan polarity scaffolds.Nature communications · 2020Article
- Review
- YFR016c/Aip5 is part of an actin nucleation complex in yeast.Biology open · 2019Article
- Comparative genomic analysis of the 'pseudofungus'Open biology · 2018Article
- What Defines the "Kingdom" Fungi?Microbiology spectrum · 2017Review
- Novel Coronin7 interactions with Cdc42 and N-WASP regulate actin organization and Golgi morphology.Scientific reports · 2016Article
- A protein complex containing Epo1p anchors the cortical endoplasmic reticulum to the yeast bud tip.The Journal of cell biology · 2015Article
- A spindle pole antigen gene MoSPA2 is important for polar cell growth of vegetative hyphae and conidia, but is dispensable for pathogenicity in Magnaporthe oryzae.Current genetics · 2014Article
- The role of Cdc42 and Gic1 in the regulation of septin filament formation and dissociation.eLife · 2013Article
- Different polarisome components play distinct roles in Slt2p-regulated cortical ER inheritance in Saccharomyces cerevisiae.Molecular biology of the cell · 2013Article
- Functional genomics in the study of yeast cell polarity: moving in the right direction.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- Role of endocytosis in localization and maintenance of the spatial markers for bud-site selection in yeast.PloS one · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In Saccharomyces cerevisiae, polarized growth depends on interactions between the actin cytoskeleton and the secretory machinery. Here we show that the Rab GTPase-activating proteins (GAPs) Msb3 and Msb4 interact directly with Spa2, a scaffold protein of the "polarisome" that also interacts with the formin Bni1. Spa2 is required for the polarized localization of Msb3 and Msb4 at the bud tip. We also show that Msb3 and Msb4 bind specifically to Cdc42-GDP and Rho1-GDP in vitro and that Msb3 and Rho GDP dissociation inhibitor act independently but oppositely on Cdc42. Finally, we show that Msb3 and Msb4 are involved in Bni1-nucleated actin assembly in vivo. These results suggest that Msb3 and Msb4 regulate polarized growth by multiple mechanisms, directly regulating exocytosis through their GAP activity toward Sec4 and potentially coordinating the functions of Cdc42, Rho1, and Bni1 in the polarisome through their binding to these GTPases. A functional equivalent of the polarisome probably exists in other fungi and mammals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.