ArticleJournal of bacteriology2005
Natural transposon mutagenesis of clinical isolates of Mycobacterium tuberculosis: how many genes does a pathogen need?
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Functional genetic variation inFrontiers in microbiology · 2023Article
- Defining Discriminatory Antibody Fingerprints in Active and Latent Tuberculosis.Frontiers in immunology · 2022Article
- The MtZ Strain: Molecular Characteristics and Outbreak Investigation of the Most SuccessfulFrontiers in cellular and infection microbiology · 2022Article
- In-depth Analysis of ISFrontiers in microbiology · 2022Article
- Label-Free Comparative Proteomics of Differentially ExpressedPathogens (Basel, Switzerland) · 2021Article
- Genetic Diversity inAnnual review of genetics · 2020Review
- Isolation and comparative genomics of Mycobacterium tuberculosis isolates from cattle and their attendants in South India.Scientific reports · 2019Article
- Integrated Multi-Omic Analysis ofFrontiers in microbiology · 2018Article
- Transposition mechanism, molecular characterization and evolution of IS6110, the specific evolutionary marker of Mycobacterium tuberculosis complex.Molecular biology reports · 2017Review
- Analysis of IS6110 insertion sites provide a glimpse into genome evolution of Mycobacterium tuberculosis.Scientific reports · 2015Article
- Article
- Marked microevolution of a unique Mycobacterium tuberculosis strain in 17 years of ongoing transmission in a high risk population.PloS one · 2014Article
- Current methods in the molecular typing of Mycobacterium tuberculosis and other mycobacteria.BioMed research international · 2014Review
- Global study of IS6110 in a successful Mycobacterium tuberculosis strain: clues for deciphering its behavior and for its rapid detection.Journal of clinical microbiology · 2013Article
- IS-seq: a novel high throughput survey of in vivo IS6110 transposition in multiple Mycobacterium tuberculosis genomes.BMC genomics · 2012Article
- Article
- Characterization of microevolution events in Mycobacterium tuberculosis strains involved in recent transmission clusters.Journal of clinical microbiology · 2011Article
- Genomic stability over 9 years of an isoniazid resistant Mycobacterium tuberculosis outbreak strain in Sweden.PloS one · 2011Article
- Novel hot spot of IS6110 insertion in Mycobacterium tuberculosis.Journal of clinical microbiology · 2010Article
- Bacterial genetic signatures of human social phenomena among M. tuberculosis from an Aboriginal Canadian population.Molecular biology and evolution · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Transposable elements can affect an organism's fitness through the insertional inactivation of genes and can therefore be used to identify genes that are nonessential for growth in vitro or in animal models. However, these models may not adequately represent the genetic requirements during chains of human infection. We have therefore conducted a genome-wide survey of transposon mutations in Mycobacterium tuberculosis isolates from cases of human infection, identifying the precise, base-specific insertion sites of the naturally occurring transposable element IS6110. Of 294 distinct insertions mapped to the strain H37Rv genome, 180 were intragenic, affecting 100 open reading frames. The number of genes carrying IS6110 in clinical isolates, and hence apparently not essential for infection and transmission, is very much lower than the estimates of nonessential genes derived from in vitro studies. This suggests that most genes in M. tuberculosis play a significant role in human infection chains. IS6110 insertions were underrepresented in genes associated with virulence, information pathways, lipid metabolism, and membrane proteins but overrepresented in multicopy genes of the PPE family, genes of unknown function, and intergenic sequences. Population genomic analysis of isolates recovered from an organism's natural habitat is an important tool for determining the significance of genes or classes of genes in the natural biology of an organism.
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