ArticleThe Journal of physiology2005
Localization of myosin II and V isoforms in cultured rat sympathetic neurones and their potential involvement in presynaptic function.
Article in The Journal of physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- Imbalance of bladder neurohomeostasis by Myosin 5a aggravates diabetic cystopathy.Molecular medicine (Cambridge, Mass.) · 2025Article
- Mechanisms of Synaptic Vesicle Exo- and Endocytosis.Biomedicines · 2022Review
- Neurotransmitter Release Site Replenishment and Presynaptic Plasticity.International journal of molecular sciences · 2020Review
- Article
- Millisecond CaProceedings of the Japan Academy. Series B, Physical and biological sciences · 2017Review
- Neural activity selects myosin IIB and VI with a specific time window in distinct dynamin isoform-mediated synaptic vesicle reuse pathways.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Myosin motors at neuronal synapses: drivers of membrane transport and actin dynamics.Nature reviews. Neuroscience · 2013Review
- Dual pools of actin at presynaptic terminals.Journal of neurophysiology · 2012Article
- Role of myosin Va in purinergic vesicular neurotransmission in the gut.American journal of physiology. Gastrointestinal and liver physiology · 2012Article
- A novel interaction of CLN3 with nonmuscle myosin-IIB and defects in cell motility of Cln3(-/-) cells.Experimental cell research · 2011Article
- Conventional myosins - unconventional functions.Biophysical reviews · 2010Review
- Nonmuscle Myosin II helps regulate synaptic vesicle mobility at the Drosophila neuromuscular junction.BMC neuroscience · 2010Article
- Myosin II motor proteins with different functions determine the fate of lamellipodia extension during cell spreading.PloS one · 2010Article
- KIF5B motor adaptor syntabulin maintains synaptic transmission in sympathetic neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Presynaptic release probability and readily releasable pool size are regulated by two independent mechanisms during posttetanic potentiation at the calyx of Held synapse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Myosin va mediates docking of secretory granules at the plasma membrane.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Myosin light chain kinase is not a regulator of synaptic vesicle trafficking during repetitive exocytosis in cultured hippocampal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While vesicle transport is one of the principal functions of myosin motors in neurones, the role played by specific myosin subtypes in discrete vesicle trafficking is poorly understood. We conducted electrophysiological and morphological experiments to determine whether myosin isoforms II and V might be involved in the transport of small synaptic vesicles in presynaptic nerve terminals of a model cholinergic synapse. Electron microscopy revealed the presence of normal synaptic architecture and synaptic vesicle density in presynaptic terminals of cultured superior cervical ganglion neurones (SCGNs) from myosin Va null rats (dilute-opisthotonus, dop). Similarly, electrophysiological analyses of synaptic transmission and synaptic vesicle cycling at paired SCGN synapses failed to uncover any significant differences in synaptic development and function between normal and dop rats. Immunocytochemistry and in situ localization of green fluorescent protein (GFP)-fusion proteins in wild-type synapses revealed that myosins IIB and Va were distributed throughout the cell soma and processes of SCGNs, while myosins IIA and Vb were not detected in SCGNs. Myosin Va was conspicuously absent in presynaptic nerve terminals, but myosin IIB alone was found to be expressed. Furthermore, synaptic transmission was inhibited by introduction of myosin IIB heavy chain fragments into presynaptic terminals of SCGNs. Together these results suggest that only myosin IIB isoform participates in vesicle trafficking in presynaptic nerve terminals of cultured SCGNs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.