ArticleImmunology2005
Cholesterol depletion inhibits src family kinase-dependent calcium mobilization and apoptosis induced by rituximab crosslinking.
Article in Immunology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 55 citations in OpenAlex.
- CaBiomolecules · 2023Review
- Cell-surface photochemistry mediated calcium overload for synergistic tumor therapy.Journal of nanobiotechnology · 2023Article
- Cyto-friendly polymerization at cell surfaces modulates cell fate by clustering cell-surface receptors.Chemical science · 2020Article
- Use of anti-CD20 therapy in follicular and marginal zone lymphoma: a review of the literature.Drugs in context · 2020Review
- Drug-free albumin-triggered sensitization of cancer cells to anticancer drugs.Journal of controlled release : official journal of the Controlled Release Society · 2019Article
- Pemphigus: Current and Future Therapeutic Strategies.Frontiers in immunology · 2019Review
- Article
- Past, Present, and Future of Rituximab-The World's First Oncology Monoclonal Antibody Therapy.Frontiers in oncology · 2018Review
- Drug-Free Macromolecular Therapeutics Induce Apoptosis via Calcium Influx and Mitochondrial Signaling Pathway.Macromolecular bioscience · 2018Article
- Drug-Free Macromolecular Therapeutics--A New Paradigm in Polymeric Nanomedicines.Biomaterials science · 2015Article
- A hybrid protein-polymer nanoworm potentiates apoptosis better than a monoclonal antibody.ACS nano · 2014Article
- Tuning innate immune activation by surface texturing of polymer microparticles: the role of shape in inflammasome activation.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Article
- Role of lipid rafts in liver health and disease.World journal of gastroenterology · 2011Review
- Article
- Review
- Rituximab sensitizes a Burkitt lymphoma cell line to cell killing by X-irradiation.Radiation and environmental biophysics · 2009Article
- Monoclonal antibodies directed to CD20 and HLA-DR can elicit homotypic adhesion followed by lysosome-mediated cell death in human lymphoma and leukemia cells.The Journal of clinical investigation · 2009Article
- Induction of apoptosis by cross-linking antibodies bound to human B-lymphoma cells: expression of Annexin V binding sites on the antibody cap.Cancer biotherapy & radiopharmaceuticals · 2009Article
- Lipid rafts and T-lymphocyte function: implications for autoimmunity.FEBS letters · 2008Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The monoclonal antibody (mAb) rituximab produces objective clinical responses in patients with B-cell non-Hodgkin's lymphoma and antibody-based autoimmune diseases. Mechanisms mediating B-cell depletion by rituximab are not completely understood and may include direct effects of signalling via the target antigen CD20. Like most but not all CD20 mAbs, rituximab induces a sharp change in the solubility of the CD20 protein in the non-ionic detergent Triton-X-100, reflecting a dramatic increase in the innate affinity of CD20 for membrane raft signalling domains. Apoptosis induced by rituximab hypercrosslinking has been shown to require src family kinases (SFK), which are enriched in rafts. In this report we provide experimental evidence that SFK-dependent apoptotic signals induced by rituximab are raft dependent. Cholesterol depletion prevented the association of hypercrosslinked CD20 with detergent-insoluble rafts, and attenuated both calcium mobilization and apoptosis induced with rituximab. CD20 cocapped with the raft-associated transmembrane adaptor LAB/NTAL after hypercrosslinking with CD20 mAbs, regardless of their ability to induce a change in the affinity of CD20 for rafts. Taken together, the data demonstrate that CD20 hypercrosslinking via rituximab activates SFKs and downstream signalling events by clustering membrane rafts in which antibody-bound CD20 is localized in a high-affinity configuration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.