Trial reportPLoS medicine2005
Marked differences in human melanoma antigen-specific T cell responsiveness after vaccination using a functional microarray.
Trial report in PLoS medicine, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed, 90 citations in OpenAlex.
- Confinement of Suspension-Cultured Cells in Polyethylene Glycol/Polyethylene Oxide-Albumin Aqueous Two-Phase Systems.Frontiers in chemistry · 2019Article
- Frequency and reactivity of antigen-specific T cells were concurrently measured through the combination of artificial antigen-presenting cell, MACS and ELISPOT.Scientific reports · 2017Article
- Evolution of MHC-based technologies used for detection of antigen-responsive T cells.Cancer immunology, immunotherapy : CII · 2017Review
- A Simple Microfluidic Platform for Long-Term Analysis and Continuous Dual-Imaging Detection of T-Cell Secreted IFN-γ and IL-2 on Antibody-Based Biochip.Biosensors · 2015Article
- The functional contribution of calcium ion flux heterogeneity in T cells.Immunology and cell biology · 2015Review
- Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus.Scientific reports · 2015Article
- Application of the pMHC Array to Characterise Tumour Antigen Specific T Cell Populations in Leukaemia Patients at Disease Diagnosis.PloS one · 2015Article
- A peripheral circulating TH1 cytokine profile is inversely associated with prostate cancer risk in CLUE II.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2014Article
- Nanowire array chips for molecular typing of rare trafficking leukocytes with application to neurodegenerative pathology.Nanoscale · 2014Article
- Advances in the study of HLA-restricted epitope vaccines.Human vaccines & immunotherapeutics · 2013Review
- The innovative evolution of cancer gene and cellular therapies.Cancer gene therapy · 2013Review
- New targets for the immunotherapy of colon cancer-does reactive disease hold the answer?Cancer gene therapy · 2013Review
- Imaging of Protein Secretion from a Single Cell Using Plasmonic Substrates.BioNanoScience · 2013Article
- Development of a hypersensitive periodate-cleavable amino acid that is methionine- and disulfide-compatible and its application in MHC exchange reagents for T cell characterisation.Chembiochem : a European journal of chemical biology · 2013Article
- Trial watch: Peptide vaccines in cancer therapy.Oncoimmunology · 2012Article
- Diversity-oriented approaches for interrogating T-cell receptor repertoire, ligand recognition, and function.Immunological reviews · 2012Review
- Proteomics-based dissection of human endoderm progenitors by differential cell capture on antibody array.Molecular & cellular proteomics : MCP · 2012Article
- Missing: a diagnostic technique to enumerate antigen-specific T cells.Critical reviews in oncology/hematology · 2012Review
- Life on a microarray: assessing live cell functions in a microarray format.Cellular and molecular life sciences : CMLS · 2012Review
- Biosensors for immune cell analysis-A perspective.Biomicrofluidics · 2012Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn contrast to many animal model studies, immunotherapeutic trials in humans suffering from cancer invariably result in a broad range of outcomes, from long-lasting remissions to no discernable effect. METHODS AND
findingsIn order to study the T cell responses in patients undergoing a melanoma-associated peptide vaccine trial, we have developed a high-throughput method using arrays of peptide-major histocompatibility complexes (pMHC) together with antibodies against secreted factors. T cells were specifically immobilized and activated by binding to particular pMHCs. The antibodies, spotted together with the pMHC, specifically capture cytokines secreted by the T cells. This technique allows rapid, simultaneous isolation and multiparametric functional characterization of antigen-specific T cells present in clinical samples. Analysis of CD8+ lymphocytes from ten melanoma patients after peptide vaccination revealed a diverse set of patient- and antigen-specific profiles of cytokine secretion, indicating surprising differences in their responsiveness. Four out of four patients who showed moderate or greater secretion of both interferon-gamma (IFNgamma) and tumor necrosis factor-alpha (TNFalpha) in response to a gp100 antigen remained free of melanoma recurrence, whereas only two of six patients who showed discordant secretion of IFNgamma and TNFalpha did so.
conclusionSuch multiparametric analysis of T cell antigen specificity and function provides a valuable tool with which to dissect the molecular underpinnings of immune responsiveness and how this information correlates with clinical outcome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.