ArticleJournal of virology2005
Inactivation of retroviruses with preservation of structural integrity by targeting the hydrophobic domain of the viral envelope.
Article in Journal of virology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Pathogenic mechanisms and vaccine development forFrontiers in microbiology · 2025Review
- Metastable Amorphous Dispersions of Hydrophobic Naphthalene Compounds Can Be Formed in Water without Stabilizing Agents via the "Ouzo Effect".The journal of physical chemistry. B · 2023Article
- Methods of Inactivation of Highly Pathogenic Viruses for Molecular, Serology or Vaccine Development Purposes.Pathogens (Basel, Switzerland) · 2022Review
- Inactivation of avian influenza viruses by hydrostatic pressure as a potential vaccine development approach.Access microbiology · 2021Article
- Inactivation of Zika Virus by Photoactive Iodonaphthyl Azide Preserves Immunogenic Potential of the Virus.Pathogens (Basel, Switzerland) · 2019Article
- Inactivation of non-enveloped virus by 1,5 iodonaphthyl azide.BMC research notes · 2015Article
- Article
- Proof-of-concept for a virus-induced obesity vaccine; vaccination against the obesity agent adenovirus 36.International journal of obesity (2005) · 2014Article
- Second generation inactivated eastern equine encephalitis virus vaccine candidates protect mice against a lethal aerosol challenge.PloS one · 2014Article
- Dengue virus photo-inactivated in presence of 1,5-iodonaphthylazide (INA) or AMT, a psoralen compound (4'-aminomethyl-trioxsalen) is highly immunogenic in mice.Human vaccines & immunotherapeutics · 2013Article
- Inactivation of Chikungunya virus by 1,5 iodonapthyl azide.Virology journal · 2012Article
- Orthogonal inactivation of influenza and the creation of detergent resistant viral aggregates: towards a novel vaccine strategy.Virology journal · 2012Article
- Article
- Photo-activation of the hydrophobic probe iodonaphthylazide in cells alters membrane protein function leading to cell death.BMC cell biology · 2009Article
- HIV-1 envelope glycoprotein-mediated fusion and pathogenesis: implications for therapy and vaccine development.Vaccine · 2008Article
- Hydrophobic inactivation of influenza viruses confers preservation of viral structure with enhanced immunogenicity.Journal of virology · 2008Article
- Application of methods for viral clearance in stem cell production.In vitro cellular & developmental biology. AnimalArticle
- Characterization of the effects of aryl-azido compounds and UVA irradiation on the viral proteins and infectivity of human immunodeficiency virus type 1.Photochemistry and photobiologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
We describe a new approach for the preparation of inactivated retroviruses for vaccine application. The lipid domain of the viral envelope was selectively targeted to inactivate proteins and lipids therein and block fusion of the virus with the target cell membrane. In this way, complete elimination of the infectivity of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) could be achieved with preservation of antigenic determinants on the surface of the viral envelope. Inactivation was accomplished by modification of proteins and lipids in the viral envelope using the hydrophobic photoinduced alkylating probe 1,5 iodonaphthylazide (INA). Treatment of HIV and SIV isolates with INA plus light completely blocked fusion of the viral envelope and abolished infectivity. The inactivated virus remained structurally unchanged, with no detectable loss of viral proteins. Modifications to envelope and nucleocapsid proteins were detected by changes in their elution pattern on reverse-phase high-performance liquid chromatography. These modifications had no effect on primary and secondary structure epitopes as determined by monoclonal antibodies. Likewise, the inactivated HIV reacted as well as the live virus with the conformation-sensitive and broadly neutralizing anti-HIV type 1 monoclonal antibodies 2G12, b12, and 4E10. Targeting the lipid domain of biological membranes with hydrophobic alkylating compounds could be used as a general approach for inactivation of enveloped viruses and other pathogenic microorganisms for vaccine application.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.