ArticleJournal of cell science2005
Direct involvement of the isotype-specific C-terminus of beta tubulin in ciliary beating.
Article in Journal of cell science, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 41 citations in OpenAlex.
- The Effects of Co-Culturing ND7/23 Sensory Neuron-like Cells and IFRS1 Schwann Cells on Myelination: A Single-Arm Nonrandomized Study.Neurology international · 2025Article
- TUBB4B is essential for the cytoskeletal architecture of cochlear supporting cells and motile cilia development.Communications biology · 2024Article
- Tubb4b is required for multi-ciliogenesis in the mouse.Development (Cambridge, England) · 2024Article
- Native doublet microtubules from Tetrahymena thermophila reveal the importance of outer junction proteins.Nature communications · 2023Article
- Exendin-4 Promotes Schwann Cell Survival/Migration and Myelination In Vitro.International journal of molecular sciences · 2021 · on this mapArticle
- The dynamic and structural properties of axonemal tubulins support the high length stability of cilia.Nature communications · 2019Article
- E-hooks provide guidance and a soft landing for the microtubule binding domain of dynein.Scientific reports · 2018Article
- Establishment of a myelinating co-culture system with a motor neuron-like cell line NSC-34 and an adult rat Schwann cell line IFRS1.Histochemistry and cell biology · 2018Article
- Quantitative Proteomic Analysis of Human Airway Cilia Identifies Previously Uncharacterized Proteins of High Abundance.Journal of proteome research · 2017Article
- The motility of axonemal dynein is regulated by the tubulin code.Biophysical journal · 2014Article
- Rho-associated kinase activity is required for proper morphogenesis of the inner cell mass in the mouse blastocyst.Biology of reproduction · 2013Article
- Displacement-weighted velocity analysis of gliding assays reveals that Chlamydomonas axonemal dynein preferentially moves conspecific microtubules.Biophysical journal · 2013Article
- Myelination in coculture of established neuronal and Schwann cell lines.Histochemistry and cell biology · 2012Article
- Particulate matter in cigarette smoke increases ciliary axoneme beating through mechanical stimulation.Journal of aerosol medicine and pulmonary drug delivery · 2012Article
- Cryo-electron tomography reveals conserved features of doublet microtubules in flagella.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Specific alpha- and beta-tubulin isotypes optimize the functions of sensory Cilia in Caenorhabditis elegans.Genetics · 2010Article
- The beta isotypes of tubulin in neuronal differentiation.Cytoskeleton (Hoboken, N.J.) · 2010Article
- Tubulin evolution in insects: gene duplication and subfunctionalization provide specialized isoforms in a functionally constrained gene family.BMC evolutionary biology · 2010Article
- Molecular basis for class V beta-tubulin effects on microtubule assembly and paclitaxel resistance.The Journal of biological chemistry · 2009Article
- Cooperativity between the beta-tubulin carboxy tail and the body of the molecule is required for microtubule function.Cell motility and the cytoskeleton · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
In previous studies in Drosophila, Nielsen et al. hypothesized that the beta tubulin C-terminal axonemal motif ;EGEFXXX', where X is an acidic amino acid, is required for ciliary function and assembly (Nielsen et al., 2001, Curr. Biol. 11, 529-533). This motif is present in some but not all mammalian beta tubulin isotypes. We therefore investigated whether this motif is important in ciliary function in mammals. In a preparation of isolated, ATP-reactivated bovine tracheal cilia, we found that monoclonal antibodies directed against the C-terminus of betaI, betaIV and betaV tubulin blocked ciliary beating in a concentration dependent manner. Antibodies against other epitopes of beta tubulin were ineffective, as were antibodies against alpha tubulin. Peptides consisting of the axonemal motif and motif-like sequences of these isotypes blocked ciliary beating. These results suggest that the axonemal motif sequences of betaI, betaIV and betaV tubulin are essential for ciliary function. Peptides consisting of corresponding C-terminal sequences in alpha tubulin isotypes were also ineffective in blocking ciliary beating, which suggests that the C-terminus of alpha tubulin is not directly involved in cilia function in mammals.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.