ArticleJournal of bacteriology2005
Extended -10 motif is critical for activity of the cspA promoter but does not contribute to low-temperature transcription.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 27 citations in OpenAlex.
- Pleiotropic roles of cold shock proteins with special emphasis on unexplored cold shock protein member of Plasmodium falciparum.Malaria journal · 2020Review
- The GTPase BipA expressed at low temperature inThe Journal of biological chemistry · 2018Article
- Cold Shock Proteins: A Minireview with Special Emphasis on Csp-family of Enteropathogenic Yersinia.Frontiers in microbiology · 2016Review
- Identification of two DNA helicases UvrD and DinG as suppressors for lethality caused by mutant cspA mRNAs.Journal of molecular microbiology and biotechnology · 2012Article
- A new regulatory circuit in ribosomal protein operons: S2-mediated control of the rpsB-tsf expression in vivo.RNA (New York, N.Y.) · 2008Article
- Production of active eukaryotic proteins through bacterial expression systems: a review of the existing biotechnology strategies.Molecular and cellular biochemistry · 2008Review
- Agr system of Listeria monocytogenes EGD-e: role in adherence and differential expression pattern.Applied and environmental microbiology · 2007Article
- Transcription initiation by mix and match elements: flexibility for polymerase binding to bacterial promoters.Gene regulation and systems biology · 2007Article
- Review
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Bacterial promoters belonging to the extended -10 class contain a conserved TGn motif upstream of the -10 promoter consensus element. Open promoter complexes can be formed on some extended -10 Escherichia coli promoters at temperatures as low as 6 degrees C, when complexes on most promoters are closed. The promoter of cspA, a gene that codes for the major cold shock protein CspA of E. coli, contains an extended -10 motif. CspA is dramatically induced upon temperature downshift from 37 to 15 degrees C, and its cold shock induction has been attributed to transcription, translation, and mRNA stabilization effects. Here, we show that though the extended -10 motif is critical for high-level expression of cspA, it does not contribute to low-temperature expression. In fact, transcription from the wild-type cspA promoter is cold sensitive in vitro and in vivo. Thus, transcription appears to play little or no role in low-temperature induction of cspA expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.