ArticleJournal of bacteriology2005
Positive autoregulation of cI is a dispensable feature of the phage lambda gene regulatory circuitry.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Bacteriophage self-counting in the presence of viral replication.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The Need for Speed: Run-On Oligomer Filament Formation Provides Maximum Speed with Maximum Sequestration of Activity.Journal of virology · 2019Article
- The role of repressor kinetics in relief of transcriptional interference between convergent promoters.Nucleic acids research · 2016Article
- Role of cis-acting sites in stimulation of the phage λ P(RM) promoter by CI-mediated looping.Journal of bacteriology · 2013Article
- Transcription-factor-mediated DNA looping probed by high-resolution, single-molecule imaging in live E. coli cells.PLoS biology · 2013Article
- Evolution of genetic switch complexity.Bacteriophage · 2013Article
- A genetic bistable switch utilizing nonlinear protein degradation.Journal of biological engineering · 2012Article
- General properties of transcriptional time series in Escherichia coli.Nature genetics · 2011Article
- Decision making in living cells: lessons from a simple system.Annual review of biophysics · 2011Review
- Lysogen stability is determined by the frequency of activity bursts from the fate-determining gene.Molecular systems biology · 2010Article
- Stability and instability in the lysogenic state of phage lambda.Journal of bacteriology · 2010Article
- The bacteriophage lambda CI protein finds an asymmetric solution.Current opinion in structural biology · 2009Review
- Binding cooperativity in phage lambda is not sufficient to produce an effective switch.Biophysical journal · 2008Article
- Cooperative DNA binding by CI repressor is dispensable in a phage lambda variant.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Identification and characterization of the immunity repressor (ImmR) that controls the mobile genetic element ICEBs1 of Bacillus subtilis.Molecular microbiology · 2007Article
- General transfer matrix formalism to calculate DNA-protein-drug binding in gene regulation: application to OR operator of phage lambda.Nucleic acids research · 2007Article
- A synthetic phage lambda regulatory circuit.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Role of the lytic repressor in prophage induction of phage lambda as analyzed by a module-replacement approach.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
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Authors and funding
2 authors.
Funding
Abstract
Complex gene regulatory circuits contain many features that are likely to contribute to their operation. It is unclear, however, whether all these features are necessary for proper circuit behavior or whether certain ones are refinements that make the circuit work better but are dispensable for qualitatively normal behavior. We have addressed this question using the phage lambda regulatory circuit, which can persist in two stable states, the lytic state and the lysogenic state. In the lysogenic state, the CI repressor positively regulates its own expression by stimulating transcription from the P(RM) promoter. We tested whether this feature is an essential part of the regulatory circuitry. Several phages with a cI mutation preventing positive autoregulation and an up mutation in the P(RM) promoter showed near-normal behavior. We conclude that positive autoregulation is not necessary for proper operation of the lambda circuitry and speculate that it serves a partially redundant function of stabilizing a bistable circuit, a form of redundancy we term "circuit-level redundancy." We discuss our findings in the context of a two-stage model for evolution and elaboration of regulatory circuits from simpler to more complex forms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.