ArticleJournal of bacteriology2005
Nitrate-dependent activation of the Dif signaling pathway of Myxococcus xanthus mediated by a NarX-DifA interspecies chimera.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
- Deep learning framework for quantifying self-organization inProceedings of the National Academy of Sciences of the United States of America · 2026Article
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- A CRISPR with roles in Myxococcus xanthus development and exopolysaccharide production.Journal of bacteriology · 2014Article
- Genetic and biochemical dissection of a HisKA domain identifies residues required exclusively for kinase and phosphatase activities.PLoS genetics · 2012Article
- DifA, a methyl-accepting chemoreceptor protein-like sensory protein, uses a novel signaling mechanism to regulate exopolysaccharide production in Myxococcus xanthus.Journal of bacteriology · 2011Article
- Article
- FrzS regulates social motility in Myxococcus xanthus by controlling exopolysaccharide production.PloS one · 2011Article
- Phosphorylation and dephosphorylation among Dif chemosensory proteins essential for exopolysaccharide regulation in Myxococcus xanthus.Journal of bacteriology · 2010Article
- Use of two-component signal transduction systems in the construction of synthetic genetic networks.Current opinion in microbiology · 2010Review
- The S helix mediates signal transmission as a HAMP domain coiled-coil extension in the NarX nitrate sensor from Escherichia coli K-12.Journal of bacteriology · 2010Article
- Isolation and characterization of a suppressor mutation that restores Myxococcus xanthus exopolysaccharide production.Microbiology (Reading, England) · 2009Article
- Structural analysis of ligand stimulation of the histidine kinase NarX.Structure (London, England : 1993) · 2009Article
- Independence and interdependence of Dif and Frz chemosensory pathways in Myxococcus xanthus chemotaxis.Molecular microbiology · 2008Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Myxococcus xanthus fibril exopolysaccharide (EPS), essential for the social gliding motility and development of this bacterium, is regulated by the Dif chemotaxis-like pathway. DifA, an MCP homolog, is proposed to mediate signal input to the Dif pathway. However, DifA lacks a prominent periplasmic domain, which in classical chemoreceptors is responsible for signal perception and for initiating transmembrane signaling. To investigate the signaling properties of DifA, we constructed a NarX-DifA (NafA) chimera from the sensory module of Escherichia coli NarX and the signaling module of M. xanthus DifA. We report here the first functional chimeric signal transducer constructed using genes from organisms in two different phylogenetic subdivisions. When expressed in M. xanthus, NafA restored fruiting body formation, EPS production, and S-motility to difA mutants in the presence of nitrate. Studies with various double mutants indicate that NafA requires the downstream Dif proteins to function. We propose that signal inputs to the Dif pathway and transmembrane signaling by DifA are essential for the regulation of EPS production in M. xanthus. Despite the apparent structural differences, DifA appears to share similar transmembrane signaling mechanisms with enteric sensor kinases and chemoreceptors.
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