ArticlePlant molecular biology2005
Size does matter: cre-mediated somatic deletion efficiency depends on the distance between the target lox-sites.
Article in Plant molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Differential CRH expression level determines efficiency of Cre- and Flp-dependent recombination.Frontiers in neuroscience · 2023Article
- Defining Gene Function in Spermatogonial Stem Cells Through Conditional Knockout Approaches.Methods in molecular biology (Clifton, N.J.) · 2023Article
- UXT in Sertoli cells is required for blood-testis barrier integrity†.Biology of reproduction · 2020Article
- A most formidable arsenal: genetic technologies for building a better mouse.Genes & development · 2020Review
- Hypothalamic loss of Snord116 recapitulates the hyperphagia of Prader-Willi syndrome.The Journal of clinical investigation · 2018Article
- Neurodevelopmental Genetic Diseases Associated With Microdeletions and Microduplications of Chromosome 17p13.3.Frontiers in genetics · 2018Review
- PDGFRβ-P2A-CreERAngiogenesis · 2017Article
- Mup-knockout mice generated through CRISPR/Cas9-mediated deletion for use in urinary protein analysis.Acta biochimica et biophysica Sinica · 2016Article
- Article
- Distinct cell-autonomous functions of RETINOBLASTOMA-RELATED in Arabidopsis stem cells revealed by the Brother of Brainbow clonal analysis system.The Plant cell · 2011Article
- Article
- Transgene excision from wheat chromosomes by phage phiC31 integrase.Plant molecular biology · 2010Article
- Generation of single-copy T-DNA transformants in Arabidopsis by the CRE/loxP recombination-mediated resolution system.Plant physiology · 2007Article
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cre/lox recombination in vivo has become an important tool to induce chromosomal rearrangements like deletions. Using a combination of Ds transposition and Cre/lox recombination in two independent experiments on chromosomes 6 and 7 of tomato, two sets of somatic deletions up to a size of 200 kb were obtained. The efficiency of somatic deletion decreased with increasing deletion size. The largest germinally transmitted deletion had a size of only 55 kb. The results show that Cre-mediated deletion in somatic cells is less efficient when the lox sites are separated over larger distances. A further drop of the deletion efficiency after germinal transmission of the larger deletions can be explained by the probable loss of genes that are of vital importance to gametophyte function. Plasmid rescue of an 8.4 kb circularised deleted DNA showed that the Cre-mediated deletion takes place in tomato as expected. Since the circular Cre-deleted DNA could only be PCR amplified in plant cells where the deletion was not complete, the double-stranded DNA circle is assumed to be instable.
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