ArticleProceedings of the National Academy of Sciences of the United States of America2005
Use of RNA interference in Drosophila S2 cells to identify host pathways controlling compartmentalization of an intracellular pathogen.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
64 citing papers in PubMed, 123 citations in OpenAlex.
- Fighting the invader: strategies against intracellular bacteria.Frontiers in pharmacology · 2026Review
- Listeria monocytogenes: from a saprophytic to a cytosolic virulent pathogen.World journal of microbiology & biotechnology · 2025Review
- Understanding the diversity and dynamics of in vivo efferocytosis: Insights from the fly embryo.Immunological reviews · 2023Review
- Mycobacterium abscessus resists the innate cellular response by surviving cell lysis of infected phagocytes.PLoS pathogens · 2023Article
- Built to last: lysosome remodeling and repair in health and disease.Trends in cell biology · 2022Review
- Cytosolic detection of phagosomal bacteria-Mechanisms underlying PAMP exodus from the phagosome into the cytosol.Molecular microbiology · 2021Review
- Predicting host dependency factors of pathogens inComputational and structural biotechnology journal · 2021Article
- Environmental RNA interference in two-spotted spider mite, Tetranychus urticae, reveals dsRNA processing requirements for efficient RNAi response.Scientific reports · 2020Article
- ESCRT puts its thumb on the nanoscale: Fixing tiny holes in endolysosomes.Current opinion in cell biology · 2020Review
- Host-Targeted Therapeutics against Multidrug Resistant IntracellularAntibiotics (Basel, Switzerland) · 2019Review
- The ESCRT and autophagy machineries cooperate to repair ESX-1-dependent damage at the Mycobacterium-containing vacuole but have opposite impact on containing the infection.PLoS pathogens · 2018Article
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- Triggered recruitment of ESCRT machinery promotes endolysosomal repair.Science (New York, N.Y.) · 2018Article
- The eIF2α Kinase Heme-Regulated Inhibitor Protects the Host from Infection by Regulating Intracellular Pathogen Trafficking.Infection and immunity · 2018Article
- Advances in Myeloid-Like Cell Origins and Functions in the Model Organism Drosophila melanogaster.Microbiology spectrum · 2017Review
- Endothelial Cells Use a Formin-Dependent Phagocytosis-Like Process to Internalize the Bacterium Listeria monocytogenes.PLoS pathogens · 2016Article
- A Dual Microscopy-Based Assay To Assess Listeria monocytogenes Cellular Entry and Vacuolar Escape.Applied and environmental microbiology · 2016Article
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- Endocytic pathway mediates refractoriness of insect Bactrocera dorsalis to RNA interference.Scientific reports · 2015Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Three genome-wide RNA interference screens were performed in Drosophila S2 cells to dissect the contribution of host processes to Listeria monocytogenes entry, vacuolar escape, and intracellular growth. Among the 116 genes identified, several host pathways previously unrecognized as playing a role in listerial pathogenesis were identified: knockdowns affecting vacuolar trafficking to and from the multivesicular body bypassed the requirement for the essential pore-forming toxin listeriolysin O in mediating escape from phagocytic vacuoles and knockdowns affecting either subunit of serine palmitoyltransferase, a key enzyme in ceramide and sphingolipid biosynthesis, enhanced the toxicity of listeriolysin O expressed in the host cell cytosol, leading to lack of appropriate toxin activity compartmentalization and host cell death. Genome-wide RNA interference screens using Drosophila S2 cells proved to be a powerful approach to dissect host-pathogen interactions.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.