ArticleProceedings of the National Academy of Sciences of the United States of America2005
Extensive 3' modification of plant small RNAs is modulated by helper component-proteinase expression.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
66 citing papers in PubMed, 1 synthesis or guideline pooled it, 152 citations in OpenAlex.
- Meta-analysis of small RNA-sequencing errors reveals ubiquitous post-transcriptional RNA modifications.Nucleic acids research · 2009Pooled it
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- Do cells use passwords in cell-state transitions? Is cell signaling sometimes encrypted?Theory in biosciences = Theorie in den Biowissenschaften · 2020Article
- Virus and Viroid-Derived Small RNAs as Modulators of Host Gene Expression: Molecular Insights Into Pathogenesis.Frontiers in microbiology · 2020Review
- Molecular and Functional Characterization of the Somatic PIWIL1/piRNA Pathway in Colorectal Cancer Cells.Cells · 2019Article
- Epigenetics in the plant-virus interaction.Plant cell reports · 2019Review
- Potato Virus Y HCPro Suppression of Antiviral Silencing in Nicotiana benthamiana Plants Correlates with Its Ability To BindJournal of virology · 2017Article
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- Studies on differential behavior of cassava mosaic geminivirus DNA components, symptom recovery patterns, and their siRNA profiles.Virus genes · 2015Article
- Nucleotide sequence-homology-independent breakdown of transgenic resistance by more virulent virus strains and a potential solution.Scientific reports · 2015Article
- Small RNAs derived from tRNAs and rRNAs are highly enriched in exosomes from both old and new world Leishmania providing evidence for conserved exosomal RNA Packaging.BMC genomics · 2015Article
- Transfer and functional consequences of dietary microRNAs in vertebrates: concepts in search of corroboration: negative results challenge the hypothesis that dietary xenomiRs cross the gut and regulate genes in ingesting vertebrates, but important questions persist.BioEssays : news and reviews in molecular, cellular and developmental biology · 2014Article
- Quantitative analysis of ribonucleoside modifications in tRNA by HPLC-coupled mass spectrometry.Nature protocols · 2014Article
- Plant miRNAome and antiviral resistance: a retrospective view and prospective challenges.Virus genes · 2014Review
- Biases in small RNA deep sequencing data.Nucleic acids research · 2014Review
- Intracellular coordination of potyviral RNA functions in infection.Frontiers in plant science · 2014Review
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA silencing is an evolutionarily conserved process in eukaryotes that represses gene expression by using 21- to 24-nt guide RNAs to mediate mRNA cleavage or translational inhibition. Plants have two distinct groups of silencing-associated small RNAs (smRNAs): the micro RNAs (miRNAs) and the small interfering RNAs (siRNAs). A recent report by Yu et al. [Yu, B., Yang, Z., Li, J., Minakhina, S., Yang, M., Padgett, R. W., Steward, R. & Chen, X. (2005) Science 307, 932-935] has shown that plant miRNAs are modified at their 3' termini with a methyl group. Here, we show that a large fraction of all silencing-associated smRNAs in tobacco are modified; this modification occurs on the 2' hydroxyl of the terminal ribose and significantly reduces the cloning efficiency of these modified smRNAs. Expression of the strong silencing suppressor P1/helper-component proteinase results in a marked decrease in the 3'-terminal modification of viral siRNAs but does not significantly affect the modification of endogenous miRNAs and 24-nt siRNAs. The differential modification mediated by helper-component proteinase expression implies that exogenous and endogenous smRNAs are processed through independent pathways that are isolated by subcellular compartmentalization and/or the association with distinct Dicer complexes. The degree of terminal modification may play an important role in regulating the extent to which primary smRNA signals can be amplified by RNA-dependent RNA polymerases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.